Rice Genomics and Genetics 2015, Vol.6, No.2, 1-5
1
Research Report Open Access
Genetic Diversity Study of Seed Proteins in Rice Drought Tolerant Donor
Accessions
Shafina Haque, Pritesh Roy, A. Anandan , S.Samantaray, S.K.Pradhan, O.N.Singh
Crop Improvement Division, Central Rice Research Institute, Cuttack, Orissa, India
Corresponding author email:
Rice Genomics and Genetics, 2015, Vol.6, No.2 doi: 10.5376/rgg.2015.06.0002
Received: 04 Feb., 2015
Accepted: 23 Mar., 2015
Published: 27 Mar., 2015
Copyright
©
2015
Haque et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted
use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Haque et al., 2015, Genetic diversity study of seed proteins in rice drought tolerant donor accessions, Rice Genomics and Genetics, Vol.6, No.2 1
-
5 (doi:
10.5376/rgg.2015.06.0002
)
Abstract
Twenty four rice genotypes were analyzed under drought stress for seed proteins by SDS-PAGE to estimate their genetic
diversity for the purpose of genetic improving under drought condition. The storage protein profiling banding pattern was scored
based on the number and intensity of bands. A total of 90 poly peptide bands were harvested from twenty four individuals ranging
from 14.3 kD to 99.0 kD. According to UPGMA dendrogram, 100% genetic similarities of protein profiling were exhibited between
Basmati 370, Mahulata, Naveen and CR 143-2-2. The banding profile revealed that studied genotypes varies from each other either
for total number or molecular weight or intensity of protein bands except the genotypes Naveen and CR 143-2-2. This
electrophoretically detectable protein polymorphism in rice grain can be used in further breeding proposes and variety development.
Our results suggest that, screening of these genotypes on the basis of variation in seed protein profile using SDS-PAGE could be
highly effective tool to identify drought tolerant donors with good seed storage under drought stress.
Keywords
Genetic diversity; Drought stress; Seed protein; Electrophoresis
Introduction
Rice (
Oryza sativa
L.) is the primary food source for
millions of people worldwide and accounts for nearly
21% of calorie intake globally (Maclean et al., 2002).
Rice is the only cereal which is being cultivated in
varied ecosystem of which more than 55% of the total
area belongs to irrigated ecosystem. Nearly, 75% of
the total rice production comes from irrigated
ecosystem, whereas rainfed upland and rainfed
lowland contributed only 21% and 34% of the
cropped area, respectively. Rice production is severely
affected by different abiotic stresses in rainfed
environments and drought is one of the largest factor
resulting in significant yield loss in rice.
Drought is one of the most devastating abiotic stress
reported for rice crop, particularly at its reproductive
stage (Venuprasad et al., 2009a; Lanceras et al.,
2004). At the time of anthesis, due to drought stress
and high temperature, grain filling ability and mean
kernel weight is reduced resulting in loss of total
grain yield. Development of drought tolerant
varieties and effective crop management practices
are prerequisite to maximize the rice production
under the unfavorable environment of drought.
Several studies of past have reported that the major
control of plants subject to drought is exerted mainly
by constitutive traits (Blum, 2005). Moreover,
efficient selection procedures under field condition
and elimination of undesirable genes incorporated
during breeding is required for development of
drought tolerant high yielding rice varieties (Ribaut
et al., 1997). It can be possible only by the
availability of genetic variations (Javid et al., 2004)
and their assessment for varietal improvement (Sadia
et al., 2009). Among numerous techniques available
for assessing the genetic variability and relatedness
among crop germplasm, grain storage protein
analysis represents a valid alternative and/or
improved approach to varietal identification
(Mennella et al., 1999). Grain storage protein
markers are highly polymorphic and environmental
influence on their electrophoretic pattern is limited
(Sadia et al., 2009). Therefore, grain protein profiling
based on SDS-PAGE can be employed for genetic
diversity study. The current study employed the use
of grain protein polymorphism in twenty four rice
drought tolerant donors to assess their genetic
