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Rice Genomics and Genetics 2012, Vol.3, No.4, 19
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21
seed-setting rate and its correlation coefficient was
0.98. The gametophyte generation agronomic trait
comprehensive investigation showed that the heterosis
was obvious (Figure 2) and the grain quality displayed
better appearance (Figure 3) in later autumn.
Figure 2 Performance of plant heterosis in gamete generation
Note: A: Marker-assistsed breed of
Japonica
restorer; B:
Gamete generation; C: YS-CMS
Figure 3 Performance of the appearance quality of rice in
gamete generation
Note: A: Marker-assistsed breed of
Japonica
restorer; B:
Gamete generation; C: YS-CMS
Testcross combinations derived from
“
Wanlun
422×Shuhui 527
”
as the paternal parent, 40 plants, 4
F
2
lines, among them, the fertility of 10 combinations
in 1 lines was stability, whereas, the fertility of 30
combinations the other 3 strains was separated. The
results showed that there were 1 homozygous 3
heterozygous in the F
2
molecular marker genotype. On
the other hand, from
“
Wanlun 422×Milyang 42
”
as
the paternal parent, 38 plants, 4 F
2
lines, of them, the
fertility of 10 combinations in 1 linens was stable,
when the fertility of 28 combinations in the other 3
lines was separated. The results show that there were 1
homozygous 3 heterozygous in the F
2
molecular
marker genotype.
“
Wanlun 422×Miyang 46
”
as the
male paternal, 35 plants, the fertility separated in 35
combined of 4 F
2
lines, which showed that the F
2
molecular marker genotype was heterozygous (Table 3).
The results showed that we can quickly, accurately
found the restorer gene in plants by using molecular
markers, we can select the applied plants under strict
agronomic traits, linking with the other breeding
methods to verify, which can make the restorer gene
expressed more accurately.
2 Discussion
There are some research progress in rice breeding
such as resistant cultivars (Cao et al., 2003; Zhang et
Table 2 Test results of seed-setting rate and pollen fertility of measurement gamete generation
The Range of seed-setting rate
Cross numbers
Proportion
Less than 20% (Pollen fertility less than 30%)
14
12.4%
20%~40% (Pollen fertility 30-50%)
26
23.0%
40%~60% (Pollen fertility 50-70%)
43
38.1%
60%~80% (Pollen fertility 70-90%)
21
18.6%
More than 80% (Pollen fertility more than 90%)
9
8.0%
Table 3 The fertility results of gamete generation
Situation of CMS
combined with the
paternal testcross
Paternal
F
2
strains
Paternal F
3
measured
Gamete generation
fertility test
Paternal F
2
genotype
Normal
Separation Homozygous Heterozygous
CMS×(Wanlun422×Shuhui527)
4
40
10
30
1
3
CMS×(Wanlun422×Miyang42)
4
38
10
28
1
3
CMS×(Wanlun422×Miyang46)
4
35
0
35
0
3
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