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Rice Genomics and Genetics 2012, Vol.3, No.4, 19
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24
http://rgg.sophiapublisher.com
19
An Article Open Access
Marker-Assisted Selection of Japonica Restorers used for Yinshui CMS system
Xianju Wang
1,2
, Xuelin Tan
1
, Guojun Shao
2
, Hui Ma
3
, Yajun Chen
2
, Cheng Zhang
2
, Wenqi Shang
2
1. Rice Research Institute, Yunnan Agricultural University, Kunming, 650201, P.R. China;
2. Rice Research Institute, Liaoning Province, Shenyang, 110101, P.R. China;
3. Shenyang Agricultural University, Shenyang, 110161, P.R. China
Corresponding authors email:
tanxuelin2008@163.com; shaoguojun@yahoo.com.cn;
Authors
Rice Genomics and Genetics, 2012, Vol.3, No.4 doi: 10.5376/rgg.2012.03.0004
Received: 15 Mar., 2012
Accepted:
28 Apr., 2012
Published: 02 Mar., 2012
This article was first published in Molecular Plant Breeding (Vol.8, No.2, 319-324) in Chinese, and here was authorized to translate and publish the paper in
English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the
original work is properly cited.
Preferred citation for this article:
Wang et al., 2012, Marker-Assisted Selection of Japonica Restorers used for Yinshui CMS system, Rice Genomics and Genetics, Vol.3, No.4 19
-
24 (doi:
10.5376/rgg.2012.03.0004)
Abstract
In this paper, we employed the rice variety Wanlun 422 with wide compatibility, WA-type restorer line Shuhui 527,
Miyang 46, and Miyang 42 as the parent materials, and selected the
Japonica
rice restorer line for Yinshui type cytoplasmic male
sterility (YS-CMS) from the hybrid combinations F
2
and F
4
using molecular marker-assisted selection combined with the
conventional breeding method. The results showed that the plants containing two restorer genes identified by molecular markers were
selected to make hybrid combinations with YS-CMS lines. We found that the seed-setting rate and heterosis of the hybrids were
significantly improved. The results of this paper indicate that it is feasible for development of restorers for
Japonica
YS-CMS line
based on molecular marker-assisted.
Keywords
Yinshui type cytoplasmic male sterility (YS-CMS); Japonica restorer line; Marker-assisted selection; Gamete generation
Background
CMS (Cytoplasmic Male Sterility) line is the base of
hybrid rice. At present,
Japonica
hybrid rice cytoplasm
sterile is Chinsurah Boro II type (BT-type) planted
large area on the production in the north of China.
BT-CMS is gametophyte sterility, abortion period late,
self fruitfulness at the high temperature; therefore
fertility is not stable enough
.
Using BT-CMS lines in
Japonica
hybrid rice seed production, once the
preservation measures is adverse, it is vulnerable to be
biological confounding, mechanically mixed, seriously
affected the purity of hybrid rice. Nowadays, most
BT-CMS existing the problem is low outcrossing rates,
low yield of seed, as well as poorer purity and quality.
Reducing the risk of genetic vulnerability caused by
single hybrid rice CMS cytoplasmi is also the main
direction to be focused on in recent years. Therefore,
the diversification requirements of cytoplasmic male
sterile types become more and more obvious.
The single set sterile of selfing bag in
Japonica
YS-CMS type cytoplasmic male sterile breeded by
Rice Research Institute of Liaoning Province is 100%,
abortion period is single nucleus stage, pollen types is
the typical abortion and the fertility is stable. Compared
with BT-CMS lines, YS-CMS lines of
Japonica
hybrid rice have a wide application prospect. But the
lack of support with the restoration, so YS-type
restorer lines in
Japonica
rice breeding efficiency has
become a serious problem (Chen et al., 2008; 2009).
As a result of the same period in
Indica
rice YS-CMS
lines and f WA-type male sterility abortion, the
restoring and maintaining relationship was similar.
Research results showed that the fertility restoration of
Indica
type sterile lines were controlled by two pairs
of major genes, one was located in the middle of the
long arm on the tenth chromosome (Yao et al., 1997;
Tan et al., 1998; Zhang et al., 2002), named as
Rf4
,
and the other restorer gene
Rf3
was located on the first
chromosome (Zhang et al., 1997). Theoretically, we
can screen YS-type
Japonica
restorer lines from
WA-type restorer line in rice, but WA-type restorer
line wasn’t in
Japonica
rice. Furthermore, there was
reproductive isolation between YS-type
Japonica
CMS line and
Indica
rice restorer lines (Li, 2009),
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