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Rice Genomics and Genetics 2012, Vol.3, No.3, 13
-
18
http://rgg.sophiapublisher.com
13
Research Report Open Access
Rice Blast Resistance Gene
Pi1
Identified by MRG4766 Marker in 173 Yunnan
Rice Landraces
Jinbin Li
1
, Ding Li
2
, Yiding Sun
2
, Minghui Xu
2
1. Agricultural Environment and Resources Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650205, P.R. China
2. The Key Laboratory of Biotechnology Research of Yunnan Province, Institute of Biotechnology and Genetic Resources, Yunnan Academy of Agricultural
Sciences, Kunming, 650223, P.R. China
Corresponding authors email:
xuminhui@sohu.com;
Authors
Rice Genomics and Genetics, 2012, Vol.3, No.3 doi: 10.5376/rgg.2012.03.0003
Received: 01 Feb., 2012
Accepted: 02 Mar., 2012
Published: 07 Mar., 2012
This article was first published in Molecular Plant Breeding (Vol.10, No.1, 73-79) in Chinese, and here was authorized to translate and publish the paper in
English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the
original work is properly cited.
Preferred citation for this article:
Li et al., 2012, Rice Blast Resistance Gene
Pi1
Identified by MRG4766 Marker in 173 Yunnan Rice Landraces, Rice Genomics and Genetics, Vol.3, No.3
13
-
18 (doi: 10.5376/rgg.2012.03.0003)
Abstract
Rice Blast is one of the most serious rice diseases and caused great yield losses every year in the world. It had been
proved that using resistant rice varieties would be the most effective way to control this disease; therefore, mining the resistant genes
might be important foundational work in the breeding program. In the present study, we identified the existence of the
Pi1
gene
among the tested 173 landraces collected from Yunnan Province by using the SSR marker MRG4766. The results showed that the
Pi1
gene was found in 64 landraces, accounting for 36.99% in total of 173 landraces. 33 of 102 indica landraces (32.35%) and 31 of 71
japonica landraces (43.66%) both contained
Pi1
gene. The landraces harboring the
Pi1
gene were distributed in 29 counties of 11
prefectures in Yunnan province. The distribution frequency of
Pi1
gene was higher in paddy-upland rice region in South margin of
Yunnan (41.03%) than that of in single and or double indica cropping region in south of Yunnan (32.69%). The distribution frequency
of
Pi1
gene was 35.29% in single indica and or japonica cropping region in center of Yunnan, 35.29% in japonica cropping region in
northeast of Yunnan and 33.33% in japonica cropping region in northwest highland of Yunnan.
Keywords
Yunnan rice landraces; Rice blast; SSR marker;
Pi1
gene
Background
Blast is one of the most important rice diseases in the
world. Identifying and mining the resistance genes
might be important foundational work in the breeding
program. Chen and research team collected 7l5 rice
blast from the South Central China region, and the
virulence were determinated by inoculated the rice
blast on the donor parent C101LAC of
Pil
gene and
found that for the parent C1O1LAC, the frequency of
virulence to the rice variety C101LAC was 10.35%,
which indicated that C101LAC (harboring
Pi1
) were
resistance to most of physiologic races of rice blast
fungus (
Pyricularia grisea
(Cooke) Sacc.) in Chinese
(Chen et al., 2001). In Yunnan province of China,
Utilization value of 20 rice resistance gene have
evaluated, and the results shows that seven vertical
resistance genes
Pi9, Piz-5, Pil, Pita-2, Piz, Pik-h
and
Piz-t
showed higher resistance, and those resistance
gene would be employed in indica cropping region of
Yunnan;
Pi9, Piz-5, Pi1, Pita-2, Piz, Pik-h, Piz-t, Pi12,
Pita
and
Pib
would be employed in japonica-growing
-region;
Pi9, Piz-5, Pil, Pita-2
can be employed in
indica and japonica cropping region in Yunnan
province of China (Li et al., 2005). The above results
showed that
Pi1
has broad-spectrum resistance in
Yunnan, and can be used widely. The identification
and utilization of
Pi1
is significance for blast
resistance breeding program.
The resistance gene of
Pi1
is a main effect dominant
blast resistance gene (Hittalmani et al., 2000), and is
located on the end of long arm of chromosome 2, the
genetic distance from RFLP markers RZ536 to Pi1
was 11 cM (Yu et al., 1991), and the genetic distance
from SSR markers RZ536 and NpB18l to
Pi1
were
7.9 cM and 3.5 cM, respectively (Mew et al., 1994).
Further analysis found that the genetic distance from
the RFLP marker RZ536 to
Pi1
was 9.7 cM, and
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