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Rice Genomics and Genetics 2012, Vol.3, No.2, 8
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Table 3 The screening results of near isogenic introgression lines for tolerance to low nitrogen
Donor
Total of introgression line
No. of plants with low nitrogen tolerance
Dongxiang wild rice
179
28
Chaling wild rice
142
20
Jiangyong wild rice
87
9
Guangxi wild rice
110
12
Yuanjiang wild rice Hehuatang No.2
16
3
Yuanjiang wild rice Hehuatang No.4
32
7
Yuanjiang wild rice Hehuatang No.10
15
5
06YD2
81
9
06YD5
29
6
06YD6
99
11
06YD12
102
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1.4 Screening for resistance to rice blast
892 lines were planted in Fengtian village of Kenzhi
Station in Jingguangshang where was heavy blast-prone
area for nature induced screening, there were 585 lines
with resistance to leaf blast, 502 lines with resistance
to panicle-neck blast, The line of R752 had the highest
susceptible to rice blast comparing to the control of
Ganxiang B, the results shown in Table 4.
1.5 Screening for tolerance to high temperature
The weather with continuous hot days happened from
1
st
to 18
th
, August in 2010, in region of Nanchang
City, the highest temperature reached 35
℃
and or 39
℃
,
during this period, there were 723 lines occurring in the
stages of heading and flowering, which was facilitate
to screen the resistant lines to high-temperature. Most of
lines heading under the conditions of the high
temperature exhibited low seed setting rate, generally
about 40%, some lower about 10%. Whereas 18 NIL
lines heading from 3
rd
to 12
th
August, 2010, had higher
seed setting rate of 70% to 76.3%, of which exhibited
high tolerance to high temperature.
1.6 Screening for flooding-resistance
Seedlings submerged 7 days later, then drained water
off in 5 days, field survey for counting living seedlings
was carried out. The results showed that most of the
seedlings in flooded condition were almost no growing
and dead in the beginning of the 5
th
day. Whereas the
flood-resistant seedlings were still growing in flooded
condition, after draining water off, the seedlings
become thin and green seedlings. The 157 lines with
flooding tolerance from 11 combinations had been
initially screened out.
1.7 Screening for resistance to herbicide
We had the NIL lines sown in the nursery field to be
growing at 2 leaf stage, and then uniform spraying
herbicide glyphosate isopropylamine, using Ganxiang
B as control, the number of alive seedlings were
Table 4 The screening results of near isogenic introgression lines for resistance to rice blast
Donor
Introgression line
Resistance to leaf blast
Resistance to panicle neck blast
Dongxiang wild rice
179
132
115
Chaling wild rice
142
79
70
Jiangyong wild rice
87
69
56
Guangxi wild rice
110
70
63
Yuanjiang wild rice Hehuatang No.2
16
8
7
Yuanjiang wild rice Hehuatang No.4
32
20
17
Yuanjiang wild rice Hehuatang No.10
15
9
7
06YD2
81
45
40
06YD5
29
21
18
06YD6
99
82
76
06YD12
102
50
33