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Rice Genomics and Genetics 2012, Vol.3, No.2, 8
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12
http://rgg.sophiapublisher.com
8
Research Report Open Access
Building Near-Isogenic Introgression Lines of Elite Maintainer Gangxiang B
and Identifying Some Specific Traits
Yeqing Xiao , Xiaoyan Wu , Lanxiang Hu , Wenchang Wu , Shiyou Luo , Wei Deng , Dazhou Chen
Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang, 330200, P.R. China
Corresponding authors email:
cdz288@yahoo.com.cn;
Authors
Rice Genomics and Genetics, 2012, Vol.3, No.2 doi: 10.5376/rgg.2012.03.0002
Received: 16 Dec., 2011
Accepted: 17 Jan., 2012
Published: 31 Jan., 2012
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly
cited.
Preferred citation for this article:
Xiao et al., 2012, Building Near-Isogenic Introgression Lines of Elite Maintainer Gangxiang B and Identifying Some Specific Traits, Rice Genomics and
Genetics, Vol.3, No.2 8
-
12 (doi: 10.5376/rgg.2012.03.0002)
Abstract
Wild rice is abundant with types of genetic variation formed in the long evolution of natural selection, which contains a
great number of favorable genes as well as develops a strong adaptability to the environment. Efficiently exploring the favor able
genes of wild rice would be a prerequisite to achieve a breakthrough in rice breeding program. In this research we developed a
population consisting of 892 near-isogenic introgression lines (NILs) by crossing and subsequent backcrossing by using eleven wild
rice accessions as donors came from different origins and using Gangxiang B as recipient, an elite maintainer to rice cytoplasm male
sterility of three-line hybrid system developed by Rice Research Institute of Jiangxi Academy of Agricultural Sciences. There are
significant differences of phenotypic traits existing in the populations of the NILs, mainly including Growth period, plant height,
tillering ability, panicle shape, grain shape, awn etc. We preliminary identified a batch of materials using the near-isogenic introgression
lines we developed in this research, such as materials with drought resistance or high temperature resistance screened under the
conditions of drought or high temperature above 35
℃
, materials with low nitrogen tolerance or blast resistance identified under the
conditions of low nitrogen levels or in the prone areas of rice blast, as well as materials with seedling flooding tolerance or
herbicide-resistance assessed under the conditions of flooding or spraying of the herbicides such as phosphine glycosides. Using of
these excellent materials, we will attempt to pyramid the favorable traits to further improve the features of stress resistance, low
nitrogen tolerant capacity and disease resistance in the maintainer of Gangxiang B in order to lay a foundation to achieve the goal of
green super rice breeding. In the scanning outcome of large-scale NILs, most of the targeted traits could be identified in the progenies
derived from the donors of wild rice, indicating that there should be many favorable genes existing in wild rice.
Keywords
Rice (
Oryza sativa
L.); Wild rice (
Oryza rufipogon
L.); Near-isogenic introgression line; Evaluation of target trait;
Maintainer of three-line hybrid rice
Background
Agronomic wild plants are not only very important
wealth of human beings, but also important genetic
resources for human survival and development. Since
wild rice has been found in 1917, China made
remarkable achievements across the world for utilizing
the wild rice germplasms in the research. As far back
as 1929, Prof. Ding Ying bred Zhongshan No.1 and
its series of derivatives by crossing between cultivated
rice and wild rice. In 1970s, Chinese rice breeders
were the first to realize the matching sets of indica
three-line hybrid rice through transferring the cytoplas-
mic male sterility gene of common wild rice into
cultivated rice. Increase of rice yield and resistance
is benefits from the discovery and utilization of
germplasm resources. In the long term process of
natural selection, wild rice forms a rich variation of
types of which led to stronger resistance to pests and
diseases as well as strong environmental adaptability,
such as excellent gens conferring resistant to leaf
blight, rice blast, sheath blight, and plant hoppers as
well as tolerant to drought, cold, high temperature,
salinity and flooding, and so on. Therefore, wild rice
germplasms have important values for enriching genetic
basis of cultivated rice varieties to provide excellent
agronomic genes conferring resistance to pests and
other abiotic stresses, as well as to improved varieties
of cultivated rice (Li, 2005; Luo, 2005; Xu et al.,