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Table 1 Primers for the OsSPL genes and the polymorphic loci for genotyping of 171 accessions in the USDA rice mini-core collection
Primer Forward primer (5'
-
3')
Reverse primer (5'
-
3')
Annealing temperature
Locus Identifier
No. of polymorphic loci
Total loci
SPL1 ACATCCTGTAACCAAACGTTTACT
ACGAAAGTGCAGTAGAGGGGCC
56
LOC_Os01g18850
3
4
SPL2 CGCCGCAGGAGACGAAGT
GCTGGCACCCGAACAACG
61
LOC_Os01g69830
17
19
SPL3 TCTGCTGTGCTCCTTTTCTCTGG
CGATTGGCTGCTACCACCGGT
58
LOC_Os02g04680
4
4
SPL4 GTGCCAGGTGGAAGGGTG
GGGCGTAGATAGGGGTTTG
55
LOC_Os02g07780
15
15
SPL5 ACACAGTCAATAACTCACCTCA
GTTGCAGTCGCCGCCGTTGA
58
LOC_Os02g08070
5
5
SPL6 AGTTGCCGCCGACGTTTAGCA
TGCATGCTTTGGCATGCCTCCA
60
LOC_Os03g61760
2
2
SPL7 AGTAGGTCCTTCTGGCTGTCT
AATGCTGATGGGCTTTGG
49
LOC_Os04g46580
8
9
SPL8 TGCGTGAGTAGTGAGGGATA
CGTGAAGTGGCTGTAGGG
52
LOC_Os04g56170
12
13
SPL9 AGGGGCCTTCTTTATCCATGTC
GGGCAAGTGGGTGCTGTCCTAG
58
LOC_Os05g33810
0
1
SPL10 GCACTGCCACGATGCGACGAT
GGAGCTCACCTGCATGTGGTCT
59
LOC_Os06g44860
7
8
SPL11 AGGAGTACTACCGCAAGCACCG
GGCAAGGGGCAGAGAACCTTGTG
59
LOC_Os06g45310
10
11
SPL12 ACACCCCGAAGGTCCTCAACCA
ACCAAGGCAGGAAGTCCAAAAA
56
LOC_Os06g49010
8
9
SPL13 CCGTTTGTTCCAGCAGGGTGGT
TGAACAAGGCCAGGCCAGCG
59
LOC_Os07g32170
7
8
SPL14 TGCACGGTCTCAAGTTCGGCA
ATGCAAAGCGGCAGGCAGGC
60
LOC_Os08g39890
9
9
SPL15 CCCATCCGTTCGGTAGAC
GAGCCATCCAAGTTCGTTTA
52
LOC_Os08g40260
1
1
SPL16 TATGACCGTCTGCTTCCTCT
GTGCTGGCTTGTAATGGC
52
LOC_Os08g41940
5
6
SPL17 TTTGGTTCATCAGAAATCCC
GCCATAGCGAGAAGAAAGAG
52
LOC_Os09g31438
8
9
SPL18 GGAGCTAATGCGCGCCTCCA
GCATCCCACCAGCCTGTTGCT
59
LOC_Os09g32944
6
6
SPL19 ATCGCCCCACCATGGGCATT
AATTCCTTGGCATCAAACACA
56
LOC_Os11g30370
1
1
Total
128
140