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and the ligated products were transformed into the
competent cells of
E. coli
DH5α. The transformants
were characterized by colony PCR and plasmid PCR.
The positive recombinants were sequenced by Sangon
Biotech (Shanghai) Co. Ltd.
3.2.6 Sequences analysis
The sequence homology analysis was carried out by
online BLAST at NCBI (http://blast.ncbi.nlm.nih.gov/).
The sequences were submitted to Genbank database
with Sequin software.
Authors’contributions
FBY was the person who took charge of this project,
including experiment design, data analysis, writing
and modifying of the manuscript. LF conducted this
experiment.
Acknowledgements
This study was financially supported by the program
for young teachers in university and college of Henan
province (2010GGJS-075). Authors appreciate for the
advice and revising comment by the reviewers.
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