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Plant Gene and Trait, 2013, Vol.4, No.20, 109
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weight (Hossain and Fujita, 2010).
3.7 Determination of protein
The protein content of each sample was determined
following the method of Bradford (1976) using bovine
serum albumin (BSA) as a protein standard.
3.8 Statistical analysis
The experiment data were expressed as mean ±
standard deviation (SD) of three replicates. The
statistical significance of the means between the
control and the treatments were evaluated by one-way
ANOVA using the least significant difference (LSD)
test at the 1% (
P
<0.01) probability level.
Author’s contributions
Mohammad Anwar Hossain participated in the design of the experiment,
data collection, data analysis and manuscript preparation. Masayuki Fujita
supervised the experimental work and critically read the manuscript. All
authors read and approved the final manuscript.
Acknowledgement
Financial grant from Japan government (Monbukagakusho) is gratefully
acknowledged.
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