Basic HTML Version
Molecular Plant Breeding 2014, Vol.5, No.3, 10
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17
http://mpb.biopublisher.ca
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Table 1 Calculated parameters for RAPD and EST
-
SSR primers
S.No.
Primer
NB
NPB
NMB
NUB
PPB
PIC 2fi (1
-
fi)
Marker index
Resolving power
RAPD primers
1
OPA
-
02
17
15
2
0
88.2
0.33
4.37
6.984
2
OPA
-
03
16
13
3
1
81.2
0.26
2.75
4.982
3
OPA
-
05
13
13
0
1
100
0.28
3.64
5.024
4
OPA
-
07
19
15
4
3
78.9
0.21
2.49
3.882
5
OPA
-
09
17
14
3
0
82.4
0.36
4.15
6.219
6
OPA
-
10
23
21
2
6
91.3
0.31
5.94
9.916
7
OPA
-
11
17
15
2
2
88.2
0.21
2.78
4.440
8
OPB
-
10
17
17
0
1
100
0.35
5.95
8.660
9
OPC
-
02
15
15
0
2
100
0.39
5.85
9.750
10
OPC
-
08
22
19
3
1
86.4
0.36
5.90
9.978
11
OPD
-
07
18
17
1
1
94.4
0.28
4.50
5.639
12
OPD
-
18
19
17
2
1
89.5
0.30
4.56
7.470
13
OPE
-
01
13
12
1
1
92.3
0.20
2.22
3.154
14
OPE
-
02
15
15
0
1
100
0.32
4.80
6.487
15
OPE
-
03
19
18
1
0
94.7
0.39
6.65
10.714
Average
17.3
15.7
1.6
1.4
91.2
0.303
4.44
6.89
EST
-
SSR primers
1.
JU2
3
3
0
0
100
0.210
0.63
0.594
2.
JU7
3
2
1
0
60
0.321
0.63
0.140
3.
JU9
5
5
0
0
100
0.313
1.57
0.074
Average
3.66
3.33
0.33
0
86.6
0.281
0.94
0.269
Note: NB: Number of bands; NPB: Number of polymorphic bands; NMB: Number of monomorphic bands; NUB: Number of unique
bands; PPB: Percentage of polymorphic bands; PIC: Polymorphic information content
Figure 2 Cluster analysis based on molecular data
sub-clusters having linkage distance of 9.2U. The
second major cluster is grouped in 2 sub-clusters at
linkage distance of 8.8 U which are further sub
divided in many sub-sub clusters. All
B. juncea
genotypes were clustered together in one group which
further showed diversity among themselves as
represented in Figure 2.
Discussion
Characterization and evaluation of available genetic
resources is the first and foremost requirement to
ameliorate any crop. In the present investigation
RAPD markers proved to be more efficient as
compared to EST-SSRs. Since limited number of
EST-SSRs were used and they were evaluated on
agarose gel that may be the reason of getting less
polymorphism, further, variation among genic portion
is least considered to non-genic that may be
attributing to least polymorphism. Primers OPE-03,
OPC-08, OPB-10 and OPA-10 have shown remarkable
potential to discriminate genotypes on the basis of
amplified products generated, calculated parameters
and high polymorphism percentage. The polymorphism
percentage obtained in this study is comparable to