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Maize Genomics and Genetics 2012, Vol.3, No.1, 1
-
5
ISSN 1925-1971 http://mgg.sophiapublisher.com
1
Research Report Open Access
Transformation of
GBSS
Ⅰ
Gene Driven with Barley Endosperm-Specific
Promoter into Maize Inbred Line Mediated by
Agrobacterium
Rongxi Zhou , Ying Wu , Hongda Zou , Shengzhong Su , Shipeng Li , Xiaohui Shan ,
Hongkui Liu , Yaping Yuan
College of Plant Science, Jilin University, Changchun, 130062, P.R. China
Corresponding author email:
yapingyuan@yahoo.com.cn;
Authors
Maize Genomics and Genetics, 2012, Vol.3, No.1 doi: 10.5376/mgg.2012.03.0001
Received: 01, Dec., 2011
Accepted: 27, Dec., 2011
Published: 02, Jan., 2012
This article was first published in Molecular Plant Breeding (Regular Print Version) in Chinese, and here was authorized to redistribute in English under the terms of the Creative
Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Zhou et al., 2012, Transformation of
GBSS
Ⅰ
Gene Driven with Barley Endosperm-Specific Promoter into Maize Inbred Line Mediated by
Agrobacterium
, Maize Genomics
and Genetics, Vol.3, No.1, 1-5 (doi: 10.5376/mgg.2012.03.0001)
Abstract
In present study, We made a plant expression construct that ligated
GBSS
Ⅰ
cloned from common wheat and endosperm
specific promoter HorD from barley into pCAMBIA3301 by using the two bridge vector pGM-T-HorD and pGM-T-
GBSS
Ⅰ
, then
the construct named pHorD-
GBSS
Ⅰ
was transformed into EHA105. Somatic embryogenesis of inbred line Y423 was used to be
transformed by
Agrobacterium Tumefaciens
mediation. Regenerated plants were harvested to be genetically detected and total of 35
palnrts were positive by PCR accreditation, accounting for 15%. We thought this study might provide a molecular way to improve
the quality of maize starch.
Keywords
Maize (
Zea mays
L.); Granule bound starch synthase
Ⅰ
(
GBSS
Ⅰ
); Barley endosperm-specific promoter (
HorD
);
Agrobacterium
-mediated transformation
Background
Maize (
Zea mays
L.), one of the world's three major
grain crops, is the most important forage crop and
plays an important role in world food production.
Starch is the main accumulation of maize grain
material, which accounted for about 70% of grain
weight in maize. Usually, the cornstarch is referring to
the mixtures of amylose and amylopectin (Xia et al.,
2011). Normal cornstarch contains about 28% amylase
and 72% amylopectin. High amylose corn generally
refers to the corn that the proportion of amylose
content in the total corn starch reached more than 50%
(Zhang et al., 2005). Amylose is an important
industrial raw material and is widely used in food,
medicine, chemical industry, papermaking, textile,
architecture, petrochemical industry and plastics
industries and other fields (Wang et al., 2002).
Granule-bound starch synthase
Ⅰ
(
GBSS
Ⅰ
) gene, also
called the
waxy
(
Wx
) gene, is a key enzyme of
amylose synthesis.
GBSS
Ⅰ
specifically binds to the
maize endosperm starch granules, thus it can maintain
the synthesis amylose in an unbranched state.
GBSS
Ⅰ
is the only dynamic protein which can combine with
the developing starch granule. Under the conditions
of lacking branching enzyme,
GBSS
Ⅰ
only can
synthesize linear glucan (Villand et al., 1992). With
the antisense inhibition of expression of granule-
bound starch synthase
Ⅰ
(
GBSS
Ⅰ
), potato plants with
no amylose had been successfully obtained (Zhang et
al., 2010). Utilization of antisense RNA technology,
the expression of
GBSS
Ⅰ
gene can be specifically
inhibited, and the
GBSS
Ⅰ
enzyme activity was
reduced, which led the degradation of amylose content
in plant starch (Visser et al., 1991). By analyzing the
molecular biology and function of
GBSS
Ⅰ
gene in
other crops, we came to the similar conclusion that
the
main function of
GBSS
Ⅰ
was responsible for the
plant amylose synthesis (Chai et al., 2005). Barley
hordein has a high polymorphism, the main function is
to store protein, and it is synthesized in barley seeds.
Experiment results show that barley hordein promoter
HorD
is a specific promoter for endosperm, it can be
specifically expressed in the monocotyledon endo-
sperm (Pistón et al., 2007).