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Legume Genomics and Genetics 2012, Vol.3, No.2, 8
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identification of the ethanol extract of
Melia azedarach
bark. This current work plays a great significance on
the development for new bio-pesticide of dodder
control.
3 Methods and Materials
3.1 Preparation of plants ethanol extracts
Three plants,
Melia azedarach
bark and leaves,
Eucalyptus robusta
leaves,
Sapium sebiferum
leaves
were collected from campus in Guangxi University.
The materials were left to dry at room temperature and
smashed respectively, and then 500 g from each were
taken and macerated in a blender with 95% alcohol in
a ratio of 1: 10 w/v. The mixture was allowed to stand
24 hours under refrigeration. After filtration, extracts
were evaporated to dryness under pressure of 0.5~0.7
at 80
℃
. The dry extracts were dissolved in water to
the desired concentrations: 0.00 g, 0.01 g, 0.05 g, and
0.25 g dry weight material per milliliter of water.
3.2 Controlled experiment of plant extracts to dodder
The host soybean was sowed in the internship base of
Forestry College of Guangxi University. 10 cm in
length stem of dodder picked from two-year
Duranta
repens
cv. golden leaves was inoculated to soybean,
when the soybean seedlings reached 40 cm in height.
After dodder parasitized successfully, extracts in
different concentrations were sprayed on the dodder
and soybean. Each concentration repeated five times,
while the control group was treated by distilled water.
Fifteen days later, treatment groups were evaluated.
Dodder control was visually estimated on a scale of 0
(for no effect) to 100 (for 100% effectiveness). The
same scale was used to evaluate treatment injury to
soybean foliage. Dodder and soybean fresh weights
were taken for each treatment. All statistical analyses
and tests were conducted according to the methods
designated by Habib and Abdul Rahman (1988).
3.3 Protection enzymes activity of soybean seedling
assay
Protection enzymes activity was assayed, when the
soybean seedlings were treated by extracts after
fifteen days. POD activity was determined with the
quaiac-based phenol colorimetric method. CAT activity
was measured with ultraviolet spectrometry. SOD
activity was measured with NBT illumination method
(Chen and Wang, 2006; Zheng, 2006).
3.4 Data analysis
Experiment data were analyzed with software of Excel
and SPSS17.0; all data were expressed as the average
± standard error. Multiple comparisons were done
with Duncan new multiple range method, and there
was no significant difference (p<0.05) among the
same letters after the same column by Duncan test.
Authors’ Contributions
JW and JX analyzed the data and wrote the manuscript. ZDY
conceived and designed the experiment as well as modified the
manuscript. JW, JX, MYY, QHQ and SFZ preformed the
experiment. All authors have read and approved the final
manuscript.
Acknowledgments
This work was supported by National College Student Innovation
Experiment Project from Guangxi University (101059331)
and Scientific Research Funds of Guangxi University
(XB2100153).
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