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Legume Genomics and Genetics 2012, Vol.3, No.2, 8
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Research Report Open Access
Effects of Three Plant Extracts on Growth and Development of Dodder and
Soybean and on Protective Enzymes of Host
Jing Wan
1*
, Jun Xu
1*
, Mingyan Yang
1
, Zhende Yang
1
, Qinghe Huang
1
,
Shufang Zhao
2
1. Forestry College of Guangxi University, Nanning, 530004, P.R. China
2. School of Chemistry and Chemical Engineering of Guangxi University, Nanning, 530004, P.R. China
* The authors who contributed equally to this work
Corresponding authors email: dzyang68@126.com;
Authors
Legume Genomics and Genetics, 2012, Vol.3, No.2 doi: 10.5376/lgg.2012.03.0002
Received: 20 Feb., 2012
Accepted: 23 Mar., 2012
Published: 30 Mar., 2012
This article was first published in Genomics and Applied Biology in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative
Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Wan et al., 2012, Effects of Three Plant Extracts on Growth and Development of Dodder and Soybean and on Protective Enzymes of Host, Legume Genomics
and Genetics, Vol.3, No.2 8
-
13 (doi: 10.5376/lgg.2012.03.0002)
Abstract
The ethanol extracts of
Melia azedarach
bark and leaves,
Eucalyptus robusta
, leaves,
Sapium sebiferum
leaves were
studied which treated to dodder and its host soybean seedlings in different concentrations, the impact of three plants extracts on
dodder and soybean seedlings were evaluated at the 15 days after spraying. The results showed that three extracts in low
concentrations had no significant influence on the growth and development of dodder and soybean seedling. In high concentrations
(0.25 g/mL),
Eucalyptus robusta
leaves extract caused damage to soybean seedling and dodder reached at 64% and 70% respectively,
while the damage of
Melia azedarach
bark extract was 78% to dodder and only 7% to soybean seedlings. On the other hand, the
treatment of
Eucalyptus robusta
leaves and
Melia azedarach
bark extract led to superoxide dismutase (SOD) activity of soybean's
leaves rising, the highest values were 2.37 times and 2.0 times respectively as much as the control groups. But the effects in activities
of peroxidase (POD) and catalase (CAT) were different, which the highest value of POD activity caused by
Eucalyptus robusta
leaves extract was 2.28 times compared to the control group. Whereas CAT activity rose highest under
Melia azedarach
bark extract
treatment, the maximum was 1.58 times than the control group, which suggested the damage to soybean caused by
Eucalyptus
robusta
leaves extract was associated with its lower activity in CAT.
Keywords
Plant extract; Dodder (
Cuscuta chinensis
Lam.);
Melia azedarach
;
Eucalyptus robusta
;
Sapium sebiferum
; Soybean
(
Glycine max
); Host plant; Protective enzymes
Introduction
Plant is the natural treasury of active compounds, and
the secondary metabolites of plant such as flavonoid,
alkaloid, nitriles, terpenes, phytoalexin etc. played
roles in insecticidal, antibiosis and weeding (Guo,
2003). Since the 1980s, people had been aware of the
evil consequences to human being, animals and our
environment by the long-term use of chemical
pesticide, people have started to exploit biological
pesticides with the characteristic of various effects,
easily degradable, not easy for pests to produce
drug-resistance, pollution-free as well as not easy for
humans and animals to get poisoned. Biological
pesticides include pesticide, biocide, and herbicide.
Some researches showed that biological herbicide has
great application value (Habib and Abdul Rahman,
1988; Lee et al., 1997; Duke et al., 2000; Li and He,
2004; Liu et al., 2005; Gao, 2008).
Dodder,
Cuscuta chinensis
Lam
.
, belonging to
Convolvulaceae, Cuscutoideae,
Cuscuta
,
is a kind of
annual parasitic herb, whose roots and leaves are degraded;
stems can climb, and haustoria stretch into host plant’s
skin to get its nutrition (Qin, 2009, China’ s Forest Pest, 4:
10). Distributed in most part of China, dodder are
important plant quarantine objects, and its host plants exist
widely, grow rapidly, do serious long-term harm to crops
and garden plants. Since soybean is a common host plant
to dodder, in this research, we treated the dodder and
soybean seedling with the ethanol extracts of
Melia
azedarach
bark and leaves,
Eucalyptus robusta
leaves,
Sapium sebiferum
leaves in different concentrations to
study their effects on the growth of dodder and the activity
of protective enzymes in soybean, in order to provide