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International Journal of Horticulture 2014, Vol.4, No.6, 24
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filtrate will be added few drops of 0.5N alcoholic KOH.
The mixture will be observed for yellow coloration or
precipitate indicating the presence of flavonoids.
4.6.7 Phlobatanins
Deposition of a red precipitation when an aqueous
extract of the plant were boiled with 1% (v/v) aqueous
hydrochloric acid were taken as evidence for the presence
of phlobatannins (Trease and Evans, 1985).
4.7 Data Analysis
Data were subjected to analysis of variance (ANOVA)
and treatment means were separated using the New
Duncan’s Multiple Range Test. The ANOVA was
performed with SPSS 16.0 software (SPSS, Inc. 2007).
Acknowledgement
We want to thank the Government of Ekiti State, Nigeria for financial support
through the Ph.D scholarship awarded to the first author, Kayode David Ileke
tenable at the Federal University of Technology, Akure, Nigeria in 2009. We
thank Mr. Oguntokun of the Department of Animal Production and Health,
Federal University of Technology, Akure for his technical assistance during the
phytochemical screening of the plant.
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