Cotton Genomics and Genetics 2015, Vol.6, No.2, 1-7
1
Research Report Open Access
Genetic Diversity Analysis for Earliness, Fiber Quality and Cotton Leaf Curl Virus
in
Gossypium hirsutum
L. Accessions.
Frasat Saeed, Rana Husnain Shabbir, Jehanzeb Farooq , Muhammad Riaz, Khalid Mahmood
Cotton Research Institute, Ayub Agricultural Research Institute (AARI), Faisalabad, Pakistan
Corresponding author email:
Cotton Genomics and Genetics, 2015, Vol.6, No.2 doi:
10.5376/cgg.2015.06.0002
Received: 19 Mac., 2015
Accepted: 24 May, 2015
Published: 28 May, 2015
Copyright
©
2015
Farooq et al., This is an open access article published under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Saeed F, Shabbir R.S., Farooq J., Riaz M., and Mahmood K., 2015, Genetic Diversity Analysis for Earliness, Fiber Quality and Cotton Leaf Curl Virus in
Gossypium hirsutum
L. Accessions, Cotton Genomics and Genetics, Vol.6, No.2 1
-7
(doi:
10.5376/cgg.2015.06.0002
)
Abstract
In the present set of experiment 159 cotton genotypes that were imported from USA were evaluated for cotton leaf curl
virus disease tolerance, better fiber quality and various yield related traits. For evaluation of these traits cluster, principle component
and correlation analysis was employed to obtain suitable parents that can be further exploited in future breeding programmes. Days to
1stsquare had significant positive correlation with days to first flower and had positive association with nodes to 1st fruiting branch
whilst it showed negative correlation with fiber fineness. CLCuD exhibited highly significant negative correlation with plant height.
Plant height showed highly positive significant association with sympodia per plant and monopodia per plant while it showed
significant negative association with ginning out turn. The principle component analysis grouped total variation into 5 PCs in which
first two contributed 34.2% to the total variability. Regarding cluster analysis the genotypes grouped into six clusters. The genotypes
in cluster I, II and III comprised of genotypes with better earliness and fiber quality traits. Cluster IV was characterized by genotypes
having less attack of CLCuD, higher GOT% and somehow good quality of fiber traits. Cluster VI was represented by genotypes
having good plant height, sympodial branches per plant and fiber fineness. Genotypes of cluster I, II and III could be exploited in
new breeding programs because of their better earliness and fiber quality traits. Cluster IV genotypes are good to utilize for better
CLCuD tolerance and GOT% whilst cluster VI genotypes were suitable for plant height, sympodial branches per plant and fiber
fineness.
Keywords
Cotton; Cluster analysis; CLCuD%; Fiber quality; Principle component analysis
Background
T
he most widely cultivated species of the world is
Gossypium hirsutum
and it is the leading fiber crop
grown in more than 80 countries (Shakeel et al., 2011).
Cotton production in Pakistan faces the threats of both
biotic and abiotic stresses. Among the most dominant
factor limiting cotton production is cotton leaf curl
virus disease (Farooq et al., 2011). This disease first
reported in Pakistan during 1967 near Multan, since
then it drastically affected major cotton growing areas
of Pakistan. To exterminate this disease hard work has
been made to develop resistant genotypes in Pakistan.
Along with all the efforts, import of cotton leaf curl
virus resistant germplasm was also done to broaden
the genetic base which was missing in the current
germplasm in Pakistan. Plant genetic resources and
germplasm collections symbolize an important portion
of the world’s natural resources. Genetic resources and
germplasm collections are reservoirs of genes and
genotypes necessary to protect humankind of present
and future generations from emerging crop diseases
and vulnerabilities. These germplasm resources
protects mankind from future, and many times
unforeseen, crop vulnerabilities, thus protecting future
food, feed, and fiber supplies (Campbell et al,
2010).Currently different institutes are working on the
development of CLCuV resistant varieties. One major
project namely “Enhancing Cotton Germplasm,
Improving Resistant to CLCuD, Supporting Cotton
and Best Management Practices for small Farmers”
funded by USDA is running in different research
institutes of Pakistan. In this project, a lot of
germplasm was imported from United States for
