Page 6 - Triticeae Genomics and Genetics

Triticeae Genomics and Genetics 2012, Vol.3, No.1, 1- 6
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types in the present study. From figure 3, we found
that the encoded dehydrin protein sequence in the
six-rowed barley, BZ-12, was the longest, containing a
peptide enriched Gly (GYGGGVTGTGTTGTHGTG
HTT), whereas, that of BZ-26 was the shortest, and
lacking 18 amino acid residues. Among these three
types of rowed barleys, there were 63 mutation sites
totally, the lowest mutant ratio 7.94% (5/63) was
detected in the third base of genetic code, and that of
17.46% (11/63) in the second genetic code position.
However, the highest proportion of mutants 19.05%
(12/63) was taken place in the first base of genetic
code. Therefore, it was concluded that the mutation
frequency of three sites bias was differential (Table 1).
Figure 3 Amino acid alignment of DHN6 proteins from the samples and hull-less barley
Note: Sequence in black background mean 100% similarity; Sequence in blue background mean 0 similarity; “·” means absence of
nucleotides
Table 1 Mutation sites of amino acid and their genetic codes in 3 samples and hull-less barley
Types of barley Genetic codes
CUG ACG GAA GUG AGU ACC GCC GCC CGC GGC GGC BZ-12
L 68 T73 E 74 V 75 S145 T191 A209 A 215 R222 G 227 G228
CUG GCG GGA GCG GGU GCC GCC ACC CAC GAC GCC BZ-16
L 68 A73 G 74 A 75 G145 A191 A209 T 215 H222 D 227 A228
CUG GCG GGA GCG GGU GCC UCC ACC CAC GAC GCC BZ-26
L 68 A73 G 74 A 75 G145 A191 S195 T 201 H208 D 213 A214
GCU ACG GAA GUG AGU ACC GCC GCC CGC GGC GGC AF043091
A 68 T73 E 74 V 75 S145 T191 A209 A 215 R222 G 227 G228
ACC UGC CAC ACC ACC UAC ACC UAC ACC GAC BZ-12
T 234 C240 H 241 T 243 T283 Y285 T304 Y 306 T315 D 465
GCC GGC CGC GCC ACC UAC CAU UUG AUC GAC BZ-16
A 234 G240 R241 A 243 T264 Y266 H285 L 287 I294 D 430
GCC GGC CGC GCC ACC UAC CAU UUG AUC AAC BZ-26
A 220 G226 R227 A 229 T248 Y250 H269 L 271 I278 N 428
ACC GGC CAC ACC GCT UUG ACC UAC AUC AAC AF043091
T 234 G240 H 241 T 243 A264 L266 T283 Y 285 I294 N 430
Note: Six-rowed barley (BZ-12); Four-rowed barley (BZ-16); Two-rowed barley (BZ-26); Hull-less barley (AF043091)