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Triticeae Genomics and Genetics
TGG 2010, Vol.1, No.1
http://tgg.sophiapublisher.com
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Table 2 The selected primers showed stable polymorphisms in different samples
Primer name
Primer sequence
Primer name
Primer sequence
OPA-04
5'-AATCGGGCTG-3'
S21
5'-CAGGCCCTTC-3'
OPB-05
5'-TGCGCCCTTC-3'
S22
5'-TGCCGAGCTG-3'
OPD-05
5'-TGAGCGGACA-3'
S32
5'-TCGGCGATAG-3'
OPP-02
5'-TCGGCACGCA-3'
S153
5'-CCCGATTCGG-3'
OPY-01
5'-GGTGGCATCT-3'
S202
5'-GGAGAGACTC-3'
Figure 3 RAPD patterns amplified by primer OPD-05, S21 and
OPP-02 using mtDNA as template, respectively
Note: A: RAPD patterns amplified by primer OPD-05; 1: Ae.
variabilis; 2: ms (Ae. variabilis)-90-110; 3: ms (Ae.
variabilis)-90-110×5253; B: RAPD patterns amplified by
primer S21; 1: Ae. Ventricosa; 2: ms (Ae. ventricosa)-90-110; 3:
ms (Ae. ventricosa)-90-110×5253; C: RAPD patterns amplified
by primer OPP-02; 1: Ae. bicornis; 2: ms (Ae. bicornis)-90-110;
3: ms (Ae. bicornis)-90-110×5253; M: λ-EcoT14
Ⅰ
marker;
Polymorphism patterns existed between the male sterile lines
and fertility-restored F1 hybrids are marked by arrows
To verify the observed polymorphisms are indeed
derived from the mitochondrial DNA, nuclear DNA
from the CMS lines and fertility-restored F1 hybrids
was isolated and amplified using the polymorphic
primers OPY-01 and OPD-05, respectively. Results
showed that the amplified bands were same between
CMS lines and F1 hybrids. Primer OPY-01 showed no
polymorphism in male sterile line ms (Ae.
kotschyi)-90-110 and its fertility-restored hybrid ms
(Ae. kotschyi)-90-110×5253 (Figure 4A), and the
bands amplified by primer OPD-05 was also
indistinguishable between ms (Ae. variabilis)-90-110
and ms (Ae. variabilis)-90-110×5253 (Figure 4B).
3 Discussion
It is now widely believed that one of the cause of
cytoplasmic male sterility (CMS) is the functional
incompatibility between the nuclear and mitochondrial
genome. Mitochondrial mutations, such as multiple
intragenic recombination and insertion of sequences of
unknown origin, are associated with CMS (Dewey et
al., 1986; Young and Hanson, 1987; Mackenzie et al.,
1988). In most cases, CMS is associated with a variety
of mitochondrial DNA rearrangements, and no two
CMS mutations described to date have been identical
(Sandhu et al., 2007).
Figure 4 RAPD patterns amplified by primer OPY-01 and
OPD-05 using total DNA as template, respectively
Note: A and B are RAPD patterns amplified by primer OPY-01
and OPD-05, respectively; 1: ms (Ae. kotschyi)-90-110; 2: ms
(Ae. kotschyi)-90-110×5253; 3: ms (Ae. variabilis)-90-110; 4:
ms
(Ae.
variabilis)-90-110×5253;
5:
ms
(Ae.
ventricosa)-90-110; 6: ms (Ae. ventricosa)-90-110×5253; M:
DL2000 DNA marker
In the present experiment the combination of Triticum
aestivum nuclei and alien Aegilops cytoplasm resulted
in the genetic
interactions
or
functional
incompatibility between the nuclear and mitochondrial
genomes. This may cause some mutations in the
mitochondrial genome or the expression of some
genes may get suppressed due to the