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Molecular Soil Biology (online), 2011, Vol. 2 No.2, 9
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14
ISSN1925-2005
http://msb.sophiapublisher.com
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Research Letter Open Access
Cloning and Characterization of
PutSTE24
Gene from
Puccinellia tenuifolra
Which Expressed in Response to Abiotic Stresses
Meihua Zhang
1
, Lianyong Wang
1
, Linhui Dong
1
Bo Sun
1
Xinxin Zhang
1
Takano Tetsuo
2
Shenkui
Liu
1
1. Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science
Center (ASNESC), Northeast Forestry University, Harbin, 150040, China
2. Asian Natural Environmental Science Center(ANESC), University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 188-0002, Japan
Corresponding author:
shenkuiliu@nefu.edu.cn;
Authors
Molecular Soil Biology, 2011, Vol.2 No.2 doi: 10.5376/msb.2011.02.0002
Received: 03, Jun., 2011
Accepted: 20, Jun., 2011
Published: 29, Jun., 2011
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Zhang et al., 2011, Cloning and Characterization of
PutSTE24
Gene from
Puccinellia tenuifolra
Which Expressed in Response to Abiotic Stresses, Molecular
Soil Biology, Vol.2 No.2 (doi: 10.5376/msb.2011.02.0002)
Abstract
The
Puccinellia tenuifolra
cDNA library was expressed in yeast (
Saccharomyces cerevisiae
) and screened on agar plates
containing toxic concentrations of aluminum. Ninteen cDNAs were isolated that enhanced the aluminum tolerance of yeast. One
cDNA, named
PutSTE24
, has a ORF of 1 275 bp, encoding a predicted protein containing 424 amino acid, and has a high similarity
of 77% with STE24 in
Arabidopsis thilinana
.
PutSTE24
and
AtSTE24
were transformed into yeast cells separately and were treated
with AlCl
3
, salt, drought, low pH and oxidation and metal ions stresses. Results revealed that these two recombinant yeast cells
showed similarly and grew better in AlCl
3
, salt and oxidation stresses than control cells, but no obvious difference in low pH and
drought stresses. Additionally, on the responsive to the metal ions, these two genes have obvious resistance to the stresses of K
+
,
Mg
2+
and Cu
2+
, are somewhat resistant to Fe
3+
, Cd
2+
, and have no obvious responsive relationship with Ca
2+
, Mn
2+
and Ba
2+
, but to
the metal ions of Co
2+
, Ni
2+
and Zn
2+
, these two recombinant yeast cells are sensitive, growing worse than the control cells, especially
the Zn
2+
. It is basically confirmed the gene
STE24
is related to metal stresses, which has no report in the previous studies.
Keywords
Puccinellia tenuifolra
;
PutSTE24
gene; Yeast; Stresses
Background
Aluminum is a non-toxic element in the earth’s crust
at normal pH values. But in the acid soils, at the low
pH values (pH<5.5), Al
3+
is solublilized from
aluminosilicate clay minerals and is toxic to crop
plants (Kochian et al., 2004). Toxic aluminum can
disrupt a series of cellular processes, such as nutrient
acquisition, cell wall loosening, nuclear division,
cytoskeleton stability, cytoplasmic Ca
2+
homeostasis,
hormone transport and signal transduction(Matsumoto,
2000). Previous studies showed that aluminum-
activated root malate or citrate exudation from plasma
membrane or vacuolar membrane played an important
role in plant Al
3+
tolerance (Hoekenga et al., 2006).
For instance, genes
AtALMT1
and
TaALMT1
discovered
in
Arabidopsis thilinana
and wheat (
Triticum aestivum
),
that encode aluminum-dependant malate tranporters,
are the most important way to Al
3+
tolerance
(Kobayashi et al., 2007). Besides these, there are some
genes or enzymes else existing in plants, including
ZmMATE
,
OsSTARA
1/2,
AtSTOP1
,
AtBCB
(Arabidopsis
blue copper-binding protein),
parB
(tobacco glutathione
S-transferase) and catalase et al. (Satoshi et al., 2007).
The modern studies focus on the Al
3+
toxicity in acid
soils, but aluminum can be also toxic in alkali
circumstance, existing in complicated ionic ways. In
this study,
Puccinellia tenuifolra
, a typical plant in
alkali soils, was use to construct its full length cDNA
library expressed in yeast, screened the Al
3+
related
genes with AlCl
3
in the medium.
PutSTE24
, showed a
high similarity to
AtSTE24
(77%), was screened out.
The CAAX protease STE24, first identified in a
genetic screen in yeast for mutants defective in the
production of a biologically active a-mating phe-
romone, is a prenylation-dependent protease catalising