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Molecular Plant Breeding 2012, Vol.3, No.3, 26
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Continuing table 2
Functional class
Unigene ID
Putative annotation
Cell disease/denfence
gppca.CleanEST.seq.Contig64
Stress-related protein
gppca.CleanEST.seq.Contig125
Abscisic stress ripening protein
gppca.CleanEST.seq.Contig3
Heat shock protein 90
gppca.CleanEST.seq.Contig105
Pathogenesis-related protein 10
gppca.CleanEST.seq.Contig120
Glutathione S-transferase
gppca0_0006_C12.ab1
Expansin
Transport
gppca.CleanEST.seq.Contig66
Non-specific lipid transfer protein
gppca0_0005_D11.ab1
Proton-transporting ATP synthase complex, coupling factor F(o)
gppca0_0005_H09.ab1
Proton-transporting ATP synthase complex, catalytic core F(1)
gppca0_0003_B07.ab1
Di- and tricarboxylate transporters
gppca0_0012_D09.ab1
Permeases of the drug/metabolite transporter (DMT) superfamily
Photosynthesis
gppca0_0012_D04.ab1
Elongation factor Tu, chloroplastic
gppca0_0007_C04.ab1
PSII 44kDa protein
gppca0_0004_C08.ab1
Photosystem II protein K
gppca0_0013_C02.ab1
Chloroplast /photosystem II
Transcription
gppca0_0002_H07.ab1
Myb-related transcription factor
gppca0_0003_F10.ab1
Splicing factor 3a, subunit 3
Protein regulation
gppca0_0003_A01.ab1
Polyubiquitin UBQ14
gppca0_0005_H06.ab1
Ubiquitin-protein ligase
gppca0_0006_C01.ab1
SCF ubiquitin ligase, SKP1 component
Cell structure
gppca0_0008_E12.ab1
Cell cycle control protein, G10 family/nucleus
gppca.CleanEST.seq.Contig107
Cytoplasm
gppca0_0012_F12.ab1
Membrane
gppca0_0008_A12.ab1
Endoplasmic reticulum
gppca0_0007_D11.ab1
Mitochondrion
gppca.CleanEST.seq.Contig67
Cell wall
1.6 Species resource of homologous sequences
The species resource of homologous sequences were
matched in a databasederived from 24 species. The
V.
vinifera
had the best matching result with 69.1%
homologous genes.
Populus trichocarpa
showed the
second best result followed by
Ricinus communis
,
V.
hybrid cultivar
,
Nicotiana tabacum
,
V. pseudoreticulata
,
Sorghum bicolor
. The other 17 species derived from
Solanum tuberosum
,
Coffea Arabica
,
Arabidopsis
thaliana
,
Olea europaea
,
Cucumis sativus
,
Zea mays
and so on made up 2.39% of the total unigenes (Table 3).
1.7 Go classification
For gene ontology (GO) annotation, each sequence
was afforded at least one GO term. The gene with
different molecular function, biological process or cell
component was counted repeatedly and respectively.
199 genes were classified as cell component (Figure 6),
while313 genes are classified as molecular function,
Table 3 Statistics of organism origin of matched function
homologues
Latin name
No. of unigenes Percentages (%)
Vitis vinifera
491
69.15
Populus trichocarpa
6
0.85
Ricinus communis
5
0.70
Vitis hybrid cultivar
3
0.42
Nicotiana tabacum
2
0.28
Vitis pseudoreticulata
2
0.28
Sorghum bicolor
2
0.28
The others
1
2.39
and 341 as biological process (Figure 7; Figure 8).
Additionally, the number of ESTs can reflect the
copy quantities of gene expression, which relate
the proportion to the frequency of gene expression
indirectly. As shown in the three pie charts (Figure 6;
Figure 7; Figure 8), there is a high percentage of ESTs
related to cell, antioxidant activity and cellular process
in three different fields, respectively.