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Molecular Plant Breeding 2011, Vol.2, No.07, 41
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41
Research Article Open Access
Genetic Diversity of 30 Cai-xins (
Brassica rapa
var.
parachinensis
) Evaluated
Based on AFLP Molecular Data
Weidong Shi
*
, Ruikui Huang
*
, Shengmao Zhou , Faqian Xiong
The Institute of Vegetable, Guangxi Academy of Agricultural Sciences, Nanning, 530007, P.R. China
*The authors who contribute equally
Corresponding author email:
shiwd@gxaas.net;
Author
Molecular Plant Breeding, 2011, Vol.2 No.7 doi: 10.5376/mpb.2011.02.0007
Received: 18, Feb., 2011
Accepted: 12, Apr., 2011
Published: 28, Apr., 2011
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Shi et al., 2011, Genetic Diversity of 30 Cai-xins (Brassica rapa var. parachinensis) Evaluated Based on AFLP Molecular Data, Molecular Plant Breeding Vol.2
No.7 (doi: 10.5376/mpb.2011.02.0007)
Abstract
Cai-xin is common Chinese name for
Brassica rapa
var.
parachinensis
that is one of very important leafage vegetables in
South China. The objectives of this research were to detect genetic diversities of the selected 30 Cai-xins based on AFLP makers as
well as to evaluate the feasibilities of AFLP approach for biodiverse study. In this paper, the 25 pairs of AFLP primers were employed
to generate 1 160 amplified bands, of which 876 bands account for 76% were polymorphic bands The average polymorphism of
tested 25 primer combinations reached 80%, the mean of polymorphism information content (PIC) was about 0.023 9 and the
Percentage of polymorphic loci was about 85.33%. The parameters of genetic diversity were calculated by the aid of GenAlEx 6.4
software including the number of different alleles (Na) 1.754, the number of effective alleles (Ne) 1.544, Shannon’s Information
Index (I) 0.472 and He 0.363. The values of genetic distance (GD) and genetic similarity (GS) were 0.112 and 0.895, respectively.
Statistic analysis revealed that almost 100 percentage of variation existed within Cai-xins based on AMOVA data. The tested Caixins
can classified into four groups at the 0.17 threshold of Nei’s genetic distances by clustering analysis based on UPGMA approach. The
present results indicated that the genetic diversity of the tested Cai-xins should be quite low and the genetic variations of Caixins be
mostly attributed to within varieties. Whereas we confidentially have conclusions that AFLP approach might be useful, efficiency and
accuracy to detect genetic diversity among varieties, landraces and lines of Caixin, especially for those which have close relationship.
Keywords
Caixin (
Brassica rapa
var.
parachinensis
); AFLP; Genetic diversity; Polymorphism
Background
Molecular markers have been widely applied to detect
genetic diversity in
Brassica
vegetables due to the
polymorphisms of the genomic DNA at the molecular
marker level. There were some studies on the genetic
diversity of Caixin detected by the molecular makers
of AFLP, RAPD, SRAP and ISSR (Chu., 2002; Guo
et al., 2002; Zhao et al., 2005; Han, 2008; Ma, 2008;
Tan et al., 2009; Sun et al., 2010). As known, RAPD,
randomly amplified polymorphic DNA, is generated
by single, short and arbitrary oligonucleotide primer
in length of 8~10 bp, and ISSR, Inter-simple sequence
repeat polymorphism is generated with single primer
which is recognized as an abundant simple sequences
existing in genome (Tautz et al., 1984). Whereas
AFLP, as well-known molecular marker, might
uncover much more DNA polymorphism because of
the limitation of the restriction endonuclease-digestion
procedures although the procedures of polyacrylamide
gel running and silver stain are significantly elevate
the band polymorphism and segregating precision
(Blears et al., 1998). AFLP marker is a dominant
molecular marker and only needs anonymous genomic
DNA for PCR performance, the PCR results has much
more comparable with whose mentioned above at a
certain extent (Zhang and Yang, 2008). So far AFLP
has been widely used to detect genetic diversities of
Brasscia
vegetables (Sun et al., 2001; Guo et al., 2002;
Chu, 2002; Zhao et al., 2005).
Cai-xin is common Chinese name for
Brassica rapa
var.
parachinensis
that is one of very important
leafage vegetables in South China. Caixin has been
characterized based on the morphological diversities
with regard to agronomic traits including leaves,