Page 10 - Molecular Plant Breeding

Wang et al., 2011, Genetic Diversity of the Selected 64 PotatoGermplasms Revealed byAFLPMarker, Molecular Plant Breeding Vol.2 No.4 (doi: 10.5376/mpb.2011.02.0004)
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Table 2 Materials
Number CIP Number Cultivated Name Resource Number CIP Number Cultivated Name Resource
1 E5 E5 CIP 33 E107 MURCA CIP
2 E10 CCC- 1386.26 CIP 34 E108 37- 35A CIP
3 E11 ICA SIRENA CIP 35 F1 F1 CIP
4 E14 GRASO -2B CIP 36 F2 F2 CIP
5 E19 MS- 1C.2 CIP 37 F4 DTO -2 CIP
6 E20 SATURNA CIP 38 F6 CCC- 573- 9 CIP
7 E22 ANETT CIP 39 F8 F8 CIP
8 E30 YURACAINA BLANCA CIP 40 F12 F12 CIP
9 E31 CHATA BLANCA CIP 41 F13 F13 CIP
10 E32 UNKNOWN CIP 42 F14 F14 CIP
11 E33 SA-1528 CIP 43 F17 F17 CIP
12 E35 3X- 2424 CIP 44 F18 Shepody CIP
13 E37 YURACC CKANCHILLO CIP 45 F20 F20 CIP
14 E38 CUP199 CIP 46 F24 F24 CIP
15 E41 37- 35A CIP 47 F25 F25 CIP
16 E43 AGB- 69-1 CIP 48 Qingyin5 Shanxi
17 E49 2X(V-3)30 CIP 49 Xiaobaihua Gansu
18 E52 BW-I CIP 50 Favorita
19 E60 87059 CIP 51 Qingshu328 Qinghai
20 E62 VC46.5 CIP 52 Shepody
21 E73 FBA-1 CIP 53 Xiazhai65 Qinghai
22 E65 E65 CIP 54 Qingshu168 Qinghai
23 E83 E83 CIP 55 Gaoyuan4
24 E75 TUNG 4 CIP 56 Gaoyuan5
25 E76 TUNG 8 CIP 57 B1 Qinghai
26 E80 DGV -108 CIP 58 B3 Qinghai
27 E87 PARINA CIP 59 B4 Qinghai
28 E90 UNKNOWN CIP 60 B5 Qinghai
29 E94 LBGROUP III(IDIAFRIT) CIP 61 Qingshu2 Qinghai
30 E95 LB GURUP VII CIP 62 Zhongda1 Beijing
31 E99 E99 CIP 63 Leshu1 Qinghai
32 E104 ZUGUCHA CIP 64 Longshu5 Gansu
T4 DNA ligase was purchased from Fermentas. Taq
DNA polymerase, dNTPs, DL2000 and 100 bp DNA
Ladder was purchased from Tiangen Bio-technology
(Beijing) Co., Ltd.
12 pairs of primer combinations were E42M50,
E36M54, E42M48, E38M48, E35M62, E42M59,
E36M48, E39-M54, E39M59, E36M61, E36M59,
E39M49 (Table 3).
3.4 AFLP Data Analysis
Clear bands on the AFLP profile were counted as “1”,
no band counted as “0”, statistics from each pair of
primers of all bands and polymorphic bands, the
results were inputted Microsoft Excel Table.
Percentage of polymorphic bands (P): P=(K/N)×100%,
of which K is the number of polymorphic bands, N is
the number of total bands. Using NTSYS-pcVersion2.1,
according to GS=2Nij (Ni+Nj), the genetic similarity
coefficient (GS) was calculated: Ni is number of bands
of ith cultivar, Nj is number of bands of jth cultivar,
Nij is number of bands of i and j cultivars. Based on
the GS of materials, dendrogram of 64 potato germplasms
were obtained by non-weighted group average method
(Unweighted Pairgroup Average Method, UPGAM,
Sneath and Sokal, 1973). Utilizing POPGENE 32
calculated Shannon’s Information Index (I) and Nei’s