Basic HTML Version
Deng et al., 2011, Ectopic expression of an
AGAMOUS
homolog
NTAG1
from
Chinese narcissus accelerated earlier flowering and senescence in Arabidopsis, Molecular Plant Breeding Vol.2 No.3 (doi: 10.5376/mpb.2011.02.0003)
16
lines of transgenic plants showed significantly small
structure (showed by arrows in Figure 1G). Some
lines showed similar tall as Wt but with obvious
curled rosette leaves (Figure 1H). The structure of
most flowers of
35S::NTAG1
in
Col
and
Ler
in the
T
1
generation was similar as that of wild type (Wt),
Figure 1 A-C: The flower structure of Chinese narcissus; Bar=4 mm; A: The flower of Jinzhanyutai; B: The flower of Yulinglong; C:
The innerest two wholes of Yulinglong; The arrow showes the carpel; D-H: Phenotypes of transgenic Arabidopsis plants expressing
35S::NTAG1
in the T
1
generation; D,A: 14-d-old Wt
Arabidopsis
(
Ler
), Bar=3 mm; E-F, A: 14-d-old (E) and a 20-d-old (F)
NTAG1
overexpressing plant in
Ler
showed reduced size, early flowering, and small and curled leaves; Arrow shows thin and curled leave
with yellow tips, Bar=3 mm; G-H: Eighteen-d-old transgenic plants expressing
35S::NTAG1
in
Col
in the T
1
generation; Wild type
and transgenic phenotype plants segregated in this generation; All transgenic plants flowered significantly early; Some lines of
transgenic plants showed significantly small structure (showed by arrows in G) with only 1 cm tall during this stage. Some lines
showed similar tall as Wt but with obvious curled rosette leaves (H), Bar=3 mm; I: Most flowers of
35S::NTAG1
in
Col
in the T
1
generation are smaller, Bar=250 μm; J: First-whorl organs were transformed into carpelloid sepals with stigmatic papillae (showed by
arrows), Bar=2 mm; K: Compared with Wt, the
35S::NTAG1
transgenic plants flowered early (left) by producing only six-seven
small, curled rosette leaves (right), Bar=5 mm; L: The
35S::NTAG1
transgenic plants (right) began to senescence when Wt plants
(left) bolted, Bar=10 mm; M-N: Scanning electron micrographs of sepals observed in Wt (M) and
35S::NTAG1
transgenic plants (N),
Bar = 20 μm