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Molecular Plant Breeding 2011, Vol.2, No.3, 14
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14
Research Article Open Access
Ectopic expression of an
AGAMOUS
homolog
NTAG1
from Chinese narcissus
accelerated earlier flowering and senescence in Arabidopsis
Xinjie Deng
, Lijun Xiong , Yang Wang , Yue Sun , Xiaofang Li
School of Life Science, East China Normal University, Shanghai, 200062
，
China
Corresponding author email
Authors
Molecular Plant Breeding, 2011, Vol.2 No.3 doi:10.5376/mpb.2011.02.0003
Received: 28 Dec., 2010
Accepted: 06 Jan., 2011
Published: 29 Jan., 2011
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Deng et al., 2011, Ectopic expression of an
AGAMOUS
homolog
NTAG1
from Chinese narcissus accelerated earlier flowering and senescence in Arabidopsis,
Molecular Plant Breeding Vol.2 No.3 (doi: 10.5376/mpb.2011.02.0003)
Abstract
There are two cultivating varieties of Chinese narcissus, named as Yulinglong (
Narcissus tazetta
var. chinensis Roem.
Florepleno) and Jinzhanyutai (
Narcissus tazetta
var. chinensis M. Roem.) well known in China. Yulinglong plants exhibit double
flower resulted from petaloid stamens. However, the molecular basis of double flower formation is known little and unclear. Based
on the flowering ABCDE model, double flower formation is commonly used to link to C functional genes. In this present paper, the
isolation and characterization of
NTAG1
gene, an
AGAMOUS
homolog from Chinese narcissus varieties mentioned above are
reported. Sequence and expression pattern of
NTAG1
gene exhibited the same in both tested varieties. It expresses only in the
reproductive organs. Furthermore, functional analysis by using ectopic tests in Arabidopsis showed that
NATG1
might be involved in
the carpel identity and floral transition The effects of ectopic expression of
NTAG1
mainly include dwarfing, early flowering, losing
inflorescence indeterminacy,branch number increasing, and advancing senescence, whereas some of homeotic phenotypes gradually
sisappeared in higher generation of transgenic plants. The utilizations of
NATG1
gene in the future gene engineering were also
discussed in the paper.
Keywords
Chinese narcissus
AGAMOUS
homologue;
NTAG1
; Flower development; Senescence
Background
Certain flower characters including the flowering time,
floral architecture and petal color are usually
concerned by ornamental plants breeders. Double
flowers resulted from increased number of petals were
selected for their showy appearance in many
domesticated plant families. Chinese narcissus
(
Narcissus tazetta
var
.
chinensis
) is popular flower
with high cultural value in the ornamental market.But
the problem of unitary culture variety have become to
be the restrict factor for the Chinese Narcissus
industry achieving further development. There are
only two cultivating varieties,, named as Yulinglong
(
Narcissus tazetta
var. chinensis Roem. Florepleno)
and Jinzhanyutai (
Narcissus tazetta
var. chinensis M.
Roem.) respectivelly, in Chinese narcissus. The flower
of Yulinglong exhibits double flower resulted from
petaloid stamens. However, the molecular basis of the
double flower formation is known little and unclear.
And there are only a few reports about flower
formation of Chines narcissus.
The genetic mechanism regulating floral formation is
extensively studied in model plants and the ABCDE
model has been established (Bowman et al., 1989;
Parcy et al., 1998; Pelaz et al., 2001; Pelaz et al., 2000;
Roeder and Yanofsky, 2001). In this model, A and E
gene classes determine sepal identity, A, B and E
determine,petal, B, C and E specify stamen , C and E
specify carpel and D functional gene is involved in the
development of ovule identity. Functional flower
organ identity genes including.
APETALA1
(A class),
APETALA3
and
PISTILLATA
(B class),
AGAMOUS
(
AG
) (C class),
FLORAL-BINDING PROTEIN
(
FBP
)
7
and
FBP11
(D class), and
SEPALLATA1
/
2
/
3
are (E
class) have been isolated (Coen and Meyerowitz, 1991;
Colombo et al., 1997; Colombo et al., 1995; Drews et
al., 1991; Jofuku et al., 1994; Mandel et al., 1992;
Pelaz et al., 2000; Rounsley et al., 1995; Theissen,
2001; Weigel and Meyerowitz, 1993; Weigel et al.,