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rice sperm cells. RT-PCR results show that the gene is
expressed in the roots, leaves, the pollen cells and
mature pollen, pollination ovary and sperm cell with
particularly high volume in the sperm cells, thus it is a
differentially expressed genes. Miao et al (2003) used
cloning which has been expressed in the sperm cells
as a probe to screen rice sperm cell CDNA library, and
got a two full-length CDNA cloning. They then
successfully constructed expression vector PRGFP1
for
Agrobacterium
-mediated genetic transformation of
rice. In addition to building a library of cDNA to study
gene expression, the researchers also tried to introduce
foreign gene into the zygote experimentby microin-
jection of two genes (
GUS
gene, the gene regulation
of anthocyanin) were separated from the corn import
Zygote, the train after a short period of time, prove to
be imported in the homozygous gene expression in the
moment (Leduc et al., 1996).
5 Conclusion
After the model Corn fertilization succeeded, the
researchers mainly aimed to create more systems of
in
vitro
operation. At present, as an important food crop,
rice has a better foundation for embryo culture. The
culture of renewable natural homozygous plants has
also been successful. The large number of sperm cells
of rice can be isolated, but separation of egg cells in
rice quantitatively restricts the result of
in vitro
fertilized. In addition to the kinds of
in vitro
fertili-
zation, the distant vitro fertilization, the use of an egg,
a zygote with genetically engineered receptosr also
enjoyed great vitality. Whether natural or artificial
zygote , there are very high frequency splits
in vitro
,
which have incomparable advantages with a somatic
general and other cell systems.
The
in vitro
fertilization has two major advantages
which are the possibility of performing it live and with
control. we can study the process of signal transdu-
ction in vivo of fertilization and embryo which will
not be as effective if studied by physiology and bioph-
ysics methods, Additionally, we can apply biological
methods to study the process of gene expression, find
out the various aspects of the development of functional
genes, and then apply transgenic technology to change
the process of fertilization and embryo development.
If we find the mechanism that activates the egg-related
genes, there is the hope that we can use the gene to
create operating new plant type without fertilization
and embryogenesis. Its significance is self-evident.
Authors' Contributions
ZAW performed the experiment, wrote and modified the
manuscript. The author has read and approved the final
manuscript.
Acknowledgements
I thanked two anonymous reviewers for their strict criticism on
this paper.
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