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Molecular Plant Breeding 2011, Vol.2, No.16, 109
-
118
http://mpb.sophiapublisher.com
109
A Letter Open Access
Cloning of SCA Gene Related to Pollen Tube Adhesion and Oriented Growth
and Analysis of Gene Diversity in
Lilium
spp.
Yuejuan Wu , Jinteng Cui , Kezhong Zhang , Yuehui Jia
College of Landscape, Beijing Agricultural College, Beijing, 102206, P.R. China
Corresponding authors email:
zkzzxd@vip.sina.com;
Authors
Molecular Plant Breeding, 2011, Vol.2, No.16 doi: 10.5376/mpb.2011.02.0016
Received: 30 Oct., 2011
Accepted: 07 Nov., 2011
Published: 18 Jan., 2012
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Wu et al., 2011, Cloning of SCA Gene Related to Pollen Tube Adhesion and Oriented Growth and Analysis of Gene Diversity in
Lilium
spp., Molecular Plant
Breeding, Vol.2, No.16 109
-
118 (doi: 10.5376/mpb.2011.02.0016)
Abstract
Stigma/style cysteine-rich adhesin genes (
SCA
) were cloned from ‘Tresor’ (
Lilium
Asiatic Hybrids), ‘White heaven’
(
Lilium Longiflorum
Hybrids) and ‘Caruso’ (
Lilium
Oriental Hybrids) leaves by PCR approaches. In this research we obtained four
gene copies in both of ‘Tresor’ and ‘Caruse’ genomes and three gene copies in ‘White heaven’ genome. The identities of the cloned
SCA among the copies were in the ranges from 72.97% to 99.68%. There are two exons and one intron in all of cloned
SCA
sequence,
which could be deduced immature protein with 113 or 115 amino acid residues (encoding mature protein with 91 amino acid residues)
SCA protein contains an N-terminal signal peptide of typical LTP protein, eight conserved cysteine residues and two consensus
pentapeptide motifs. The identities among the amino acid sequences were in range from 87.91% to 98.90% higher than that of the
similarities of gene sequences. Alignment analysis of amino acid sequences of cloned SCAs showed there were some distinct
differences of amino acid site existing in the tested three species of lily hybrids. The phylogenetic tree revealed the relationship of the
cloned 11 amino acid sequences of SCA were all more close to LTP family of monocotyledons than that of dicotyledons, which were
consistent with the conclusions of kinship in plant taxonomy.
Keywords
Lily
(
Lilium spp.)
; Pollen tube adhesion; Directional growth;
SCA
; Gene diversity
Background
In the process of affinity pollination, the paternal
pollen germinates on the papillate cells of maternal
stigma, pollen tube goes into the style through the
papillate cells, directional growing from the top in the
style into the ovary, and then going into placenta and
micropyle from the ovary, of which pollen tube
growth precise and orderly exhibits a serial of directional
growth that would be related with the cascade
regulations of genes in different growth stages
(Higashiyama, 2010). The adhesion and directed growth
of pollen tubes in the style would be an important link
to affect whether the pollen tube could go through the
style of stigma successfully, as well as to affect
directional growth of pollen tube toward the micropylar
cap in the later growth (Palanivelu and Preuss, 2006).
Therefore, it would be helpful to partly explain the
molecular mechanism of hybridizing barriers among
the lily species by isolating the genes (
SCA
genes)
related to pollen tube adhesion and directional growth
of lily, identifying gene functions and studying the
influences of the
SCA
gene on pollen tube adhesion
and directed growth in the same species or different
species.
SCA (Stigma/style Cysteine-rich Adhesin) protein was
isolated from stigma and style secretions of Easter lily
(
Lilium longiflorum
Hybrids
) 'Nellie white' reported by
Lord’s laboratory of the University of California, of
which SCA protein could promote pollen tube
adhering to epidermis of transmitting tissue of the
style with the assistance of pectin polysaccharides as
well as promote mutual adhesion of pollen tubes
(Mollet et al., 2000, Park et al., 2000). Lord’s laboratory
also had isolated a Chemocyanin protein that promotes
pollen tube directional growth from the stigma
towards the style, while SCA protein could enhance
the function of such directional growth (Kim et al.,
2003).