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Molecular Plant Breeding 2011, Vol.2, No.15, 101
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108
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Figure 5 Multiple comparisons of rbcL amino acid sequences of different plants
Note: The plant names and accessions in Genbank of AA sequences are showed in turn as follows:
Cathaya argyrophylla
(Accession:
YP003934479),
Calycanthus floridus var. glaucus
(Accession: NP862762),
Nicotiana tabacum
(Accession: NP054507),
Solanum
lycopersicum
(Accession: YP514860),
Citrus sinensis
(Accession: ABI49028),
Arabidopsis thaliana
(Accession: NP051067),
Allium
cepa (
Accession: ABU82791),
Phalaenopsis aphrodite subsp. formosana
(Accession: YP358584),
Oryza sativa subsp. japonica
(Accession: NP039391),
Zea mays (
Accession: NC001666). The modena, pink and baby blue in the figure indicate identical,
conservative and semiconservative AA residues, respectively.
rbcL DNA sequences from
Nicotiana tabacum
,
Solanum
lycopersicum
,
Spinacia
oleracea
,
Arabidopsis thaliana
,
Citrus sinensis
,
Calycanthus
floridus var. glaucus
,
Phalaenopsis aphrodite subsp.
formosana
,
Oryza sativa subsp. japonica
,
Saccharum
officinarum
,
Zea mays
,
Cycas taitungensis
,
Cathaya
argyrophylla
,
and
Pinus thunbergii,
was performed by
Clustalx and MEGA5 software (Saitou and Nei, 1987;
Tamura et al., 2004), using Neighbor-Joining (NJ)
method. The rbcL DNA sequences, derived from
thirteen higher plants, were assembled into two big
clusters. One group is comprised of
Cycas
taitungensis, Cathaya argyrophylla, Pinus thunbergii
,
and other ten plants constitute the other group. All the
thirteen plants originate from a common ancestor. In
the phylogenetic tree, the connection of the branches
reflects the rbcLs evolutionary relationship of
different plants clearly. The three Gymnospermous
plants, containing
Cycas taitungensis, Cathaya
argyrophylla
and
Pinus thunbergii
, constitute a branch,
which is distinguished from the other branch that
include other ten plants, belonging to angiosperm.
And the ten angiospermous plants can be further
divided into two branches of dicotyledon and
monocotyledon. Also, the
Solanaceae
plant,
containing
Nicotiana tabacum
and
Solanum
lycopersicum
, and the
Poaceae
plant, including
Oryza
sativa subsp. japonica, Zea mays,
and
Saccharum