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Molecular Plant Breeding 2011, Vol.2, No.13, 92
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94
Table 1 Statistical analysis of plant height in DH population
Year
Location
No.
BS366
Baiyu149
Average
Range (cm)
CV (%)
Skewness
2007
Beijing
Funan
234
234
67
80
75
103
71
87
53~90
51~112
12
13
0.076
-
0.165
2008
Beijing
Funan
234
234
67
81
84
96
70
83
35~88
50~112
13
13
-
0.781
0.089
kinds of environmental conditions, which was
conform to normal distribution. This study implied
that plant height was controlled by multiple
quantitative trait genes, and consistent with the
requirements of QTL analysis.
1.2 Variance analysis
Variance Analysis for plant height is performed by
SPSS 16.0 software, results showed that location, year,
genotype, location and genotype interaction, and year
and genotype interaction represent significant
difference (Table 2).
Table 2 Variance analysis for plant height in DH population
Source
df
Mean square
F value
Location
Year
Replicate
Genotype
Location * genotype
Year * genotype
Error
1
1
1
233
234
234
644
31 060.633
232.067
40.649
319.139
453.031
117.36
34.035
912.6 **
12.434**
1.194
9.377 **
13.311 **
7.024**
Note: ** represent the statistic significant difference at the level
of 0.01 possibility
1.3 QTL analysis
Based on the method of composite interval mapping
(CIM), 25 QTLs of plant height, which were located
on chromosomes 1A, 1B, 2A, 2B, 2D, 3B (two), 3D
(two), 4B, 5A (two), 6A, 6B (three), 6D, 7A (three),
7B (four) and 7D were detected in four kinds of
environmental conditions, the contribution rate was
2.11%~9.64%, the additive effect was between 3.35
and 16.19.
From 2007 to 2008, a total of twenty-two QTLs have
been detected in Beijing, in which one QTL was
detected in both years. It is located on chromosome
6BL, with interval was from barc14 to gwm58, which
is closely linked to gwm58. Genetic distance was 1.93
cM, and the contribution rate was 2.11%~9.64%, and
the additive effect was between 5.67 and 9.64; A total
of twenty-two QTLs have been detected in Fu’nan. In
2007, a total of fourteen QTLs for plant height have
been detected in Beijing and Fu’nan, in which seven
QTLs were detected in both years. They are located on
chromosomes 1B, 2A, 3B (two), 3D, and 6B (two),
respectively, and explained 2.12%~9.19% of the
phenotypic variation; In 2008, a total of twelve QTLs
for plant height have been detected in Beijing and
Fu’nan, in which five QTLs were detected in both
years. They are located on chromosomes 2BS, 6BL,
7BL (two), 7DS, respectively, and the contribution
rate of single QTL was 2.87%~3.68% (Table 3).
In Beijing and Fu’nan, based on QTL analysis for
plant height in both years, there is small difference in
detected QTLs under the conditions of the different
places and the same years, and there is great
difference in detected QTLs under the conditions of
the different years and the same places (Figure 2).
QTL of located chromosome 7A has been detected in
Beijing.
2 Discussion
The results of this research indicated that all
detected QTLs for 2007 year explained 49.82% and
31.44% of the phenotypic variation in Beijing and
Fu’nan, respectively; all detected QTLs for 2008 year
explained 29.09% and 33.33% of the phenotypic
variation in Beijing and Fu’nan, respectively; this
implied that parts QTLs for plant height has not
detected. Four major reasons for above results: firstly,
constructed linkage map of wheat was not complete,
and molecular was not tightness; secondly, the
height-controlling genes in their parents are partially
identical, lacking of polymorphism; thirdly, a part of