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Molecular Plant Breeding 2011, Vol.2, No.12, 83
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Table 1 Related parameters for 26 SSR loci
Locus
N
Np
P
PCI
Ho
He
t
CAC02
3
3
100
0.445
0.288 9
0.283 9
1.008 4
CAC03
12
11
91.67
0.791
0.066 7
0.238 5
1.629 1
CAC04
5
4
80
0.423
0.577 8
0.303 1
0.441 7
CAC06
13
12
92.31
0.887
0.022 2
0.213 5
1.692 1
CAC08
4
3
75
0.448
0.177 8
0.248 2
1.236 9
CAC10
11
11
100
0.809
0.355 6
0.239 0
0.748 7
CAC11
9
8
88.89
0.683
0.266 7
0.395 3
1.584 9
CAC13
10
9
90
0.677
0.066 7
0.295 4
2.028 0
CAC20
4
2
50
0.173
0.755 6
0.673 7
0.609 6
CAC21
10
10
100
0.689
0.155 6
0.323 6
1.711 8
CAC23
2
2
100
0.299
0.266 7
0.407 5
1.672 3
CAC39
14
13
92.86
0.892
0.044 4
0.299 9
2.226 8
CAC56
5
4
80
0.401
0.222 2
0.288 6
1.236 3
CAC65
7
6
85.71
0.763
0.466 7
0.420 5
0.870 2
CAC71
4
3
75
0.497
0.288 9
0.444 9
1.838 8
CAC84
3
2
66.67
0.342
0.133 3
0.389 0
2.536 0
CN11A10
3
3
100
0.301
0.577 8
0.372 3
0.515 3
CN11E6
11
10
90.91
0.778
0.222 2
0.341 3
1.481 7
CN11E10
8
7
87.5
0.767
0.288 9
0.437 2
1.768 9
CN1G4
8
6
75
0.632
0.422 2
0.547 3
1.819 6
CN1H2
5
4
80
0.501
0.333 3
0.415 2
1.361
CN2A4
9
8
88.89
0.633
0.288 9
0.483 4
2.289 4
CN2A5
6
4
66.67
0.434
0.377 8
0.496 6
1.666 6
CNZ01
5
4
80
0.413
0.355 6
0.494 4
1.812 5
CNZ02
8
6
75
0.576
0.155 6
0.398 5
2.446 4
CNZ03
9
8
88.89
0.682
0.088 9
0.365 0
2.642 9
Total
188
163
Average
7.23
6.3
84.65
0.575
0.279 5
0.377 5
1.594 7
Note:
N
, number of amplified bands;
Np
, number of polymorphic locus;
P
, percentage of polymorphic locus;
PCI
, polymorphic
information content;
Ho
, observed heterozygosity;
He
, expected heterozygosity;
t
, apparent outcrossing rate
in the Hardy-Weinberg equilibrium, the
F
ST
should be
0.078, which corresponds to the upper value of the
confidence interval and suggests no differentiation
among plantations. The
F
IS
corresponds to the
within-population allelic correlations, which is
influenced by inbreeding. The overall value was
significantly different from zero, suggesting a slight
tendency toward inbreeding. Slight but significant
deviations from Hardy-Weinberg equilibrium indicate
the differentiation among accessions as well as
variation in the inbreeding level. However, this
differentiation could be resulted from the genetic
difference among populations or the fact that each
accession is supposed to descend from a single tree.
1.2 Bayesian assignment tests among accessions
When we applied the
Geneclass 2
assignment method
with the local accessions as the reference dataset, the
individuals of the hybrid accession from Wenchang