Basic HTML Version
Molecular Plant Breeding 2011, Vol.2, No.1, 1
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http://mpb.sophiapublisher.com
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2 Results
2.1 Genetic diversity analysis
Genetic diversity was analyzed to 55 copies of rice
lines with 53 pairs of SSR primer on rice chromosome
(Table 2). A total of 267 allelic variations have been
detected, ranged from 4 to 7, the average allelic
variation was 5.04. 7 allelic variations were examined
on RM21, RM38, RM72, RM206, RM224, RM250,
RM252 and RM528, varing from 0.287 to 0.786 with
average of PIC value at 0.624. A total of 8 pairs of
primer (RM23, RM475, RM55, RM280, RM38,
RM257, RM332 and RM19) belonged to intermediate
polymorphism (PIC: 0.25~0.50). While the rest were
ranged to high polymorphism (PIC>0.50). PIC of
RM50, RM206, RM224, RM248, RM270 and RM331
was more than 0.75. And the values of RM23 and
RM280, the lowest, were 0.345 and 0.287, respectively.
2.2 Clustering analysis and genetic relationship
According to data from 53 pairs of SSR marker,
clustering analysis and dendrogram were conducted to
55 accessions of rice with UPGMA. (Figure 1) With
threshold value setting at 0.68, 55 copies of rice could
mainly be divided into japonica rice and indica rice.
Ranged from 0.614 to 0.944, the average of genetic
similarity coefficient was 0.772. Varied from 0.685 to
0.996, the average coefficient was 0.778 for 14
accessions of japonica rice.
Similarity coefficient between two recurrent parents
and 53 accessions of donor parents was summaried in
Table 3. It indicated that the similarity coefficient
between Minghui86 and 53 accessions of parents was
0.655~0.850. The coefficient between Minghui86 and
14 copies was less than 0.7 (including 0.7). The
coefficient between Minghui86 and either Hangxiang10
or Lemont was 0.655. And the value between
Minghui86 and Teqing was 0.850, which was the
maximum. Additionally, the similarity coefficient
between Shuhui527 and 53 accessions of parent
ranged from 0.640 to 0.873. Among them, the
coefficient between Shuhui527 and 12 accessions was
less than 0.7 (including 0.7). The coefficient that the
12 accessions showed to Hangxiang10 and Han 4 was
0.640. The value of IR65600
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27
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1
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2
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2 and Minghui63
was 0.873, which was the maximum. Majority of
similarity coefficients between two recurrent parents
and japonica rices were relatively low and that of
indica rices were high. Similarity coefficients between
Shuhui527 and Indica rices were generally higher than
that of Minghui86. On the other hand, similarity
coefficients between Shuhui527 and Japonica rices
was commonly lower than that of Minghui86.
3 Discussion
Minghui86, an elite restorer line, was bred through
individual selection and testcross for eight generations
during six years between P 18 (IR54/Minghui63
∥
IR60
/Gui630) and gk148 (Japonic187/IR30
∥
Minghui63)
Table 3 Similarity coefficient matrix between recurrent and donor parent
3
4
5
6
7
8
9
10
11
12
13
14
15
16
1
0.783
0.843 0.775 0.816
0.794 0.820 0.813
0.768
0.805 0.783
0.794 0.745
0.764
0.805
2
0.790
0.873 0.820 0.824
0.802 0.835 0.835
0.820
0.835 0.805
0.816 0.738
0.824
0.813
17
18
19
20
21
22
23
24
25
26
27
28
29
30
－
0.760
0.719 0.685 0.670
0.685 0.659 0.663
0.678
0.798 0.655
0.659 0.760
0.738
0.768
－
0.820
0.734 0.663 0.640
0.678 0.659 0.663
0.655
0.805 0.640
0.674 0.760
0.738
0.805
31
32
33
34
35
36
37
38
39
40
41
42
43
44
－
0.783
0.779 0.685 0.787
0.850 0.846 0.828
0.663
0.772 0.798
0.738 0.685
0.828
0.693
－
0.798
0.824 0.708 0.802
0.828 0.831 0.873
0.663
0.787 0.813
0.745 0.700
0.850
0.678
45
46
47
48
49
50
51
52
53
54
55
－
0.742
0.655 0.787 0.787
0.805 0.749 0.805
0.802
0.670 0.775
0.730
－
0.764
0.655 0.809 0.861
0.790 0.772 0.843
0.787
0.663 0.753
0.753
Note: The number of accessions were defined in Table 1