Basic HTML Version
Molecular Plant Breeding 2010, Vol.1 No.4
http://mpb.sophiapublisher.com
Page 5 of 5
Author’s contributions
XLQ conducted the major part of this study including experimental design,
query and synthesis of markers, analysis of experimental data, and
manuscript preparation. CF participated in preliminary analysis of
experimental data and manuscript preparation. YL participated in the
development of the project and experimental design. DAM participated in
preliminary analysis of experimental data. LJ and CGL participated in
experimental preparation. HGW coordinated the project and participated in
experimental design, and manuscript preparation. All authors read and
approved the final manuscript.
Acknowledgements
Thank Yuanfeng Hao for his helpful comments on an earlier draft of this
manuscript, Xia Wu and Liming Bao for their assistance in my experiment.
And thank researcher Chen xiao for giving the wheat germplasm line
CB033 and Dr. Li Hongjie provid the E15 strains of powdery mildew. All of
the primers were inquired on GrainGenes2.0 (http:// wheat.pw.usda.gov/)
and related
References, and synthesized by Shanghai Biological
Engineering Technology Co., Ltd.; 10×PCR Buffer, dNTPs and Taq
enzymes were Takara Biotechnology Co., Ltd. products. This study was
supported in part by the Chinese Academy of Agricultural Sciences
Department. This paper is contribution from the Shandong Agricultural
University and supported by the National Natural Science Foundation of
china (30971765).
References
Cao A.Z., Wang X.E., Chen Y.P., Zou X.W., and Chen P.D., 2006, A
sequence- specific PCR marker linked with
Pm21
distinguishes
chromosome 6AS, 6BS, 6DS of
Triticum aestivum
and 6VS of
Haynaldia villosa
, Plant Breed, 125: 201-205
Chen P.D., Qi L.L., Zhou B., Zhang S.Z., and Liu D.J., 1995, Development
and molecular cytogenetic analysis of wheat-
Haynaldia villosa
6VS/6AI. translocation lines Specifying resistance to powdery mildew,
Theor. Appl. Genet., 91: 1125-1128
Chen X., Shi A.N., and Shang L.M., 1997, The resistance reaction of
h.villosa
to powdery mildew isolates and its expression in wheat
background, Acta Phytopathologica sinica, 27(1): 17-22
Chen Y.P., Wang H.Z., Cao A.Z., Wang C.M., and Chen P.D., 2006,
Cloning of a resistance gene analog from wheat and development of a
co-dominant PCR marker for
Pm21
, J. Integr. Plant Biol., 48: 715-721
Devos K.M., Millan T., and Gale M.D., 1993, Comparative RFLP maps of
the homeologous group-2 chromosomes of wheat, rye and barley,
Theoretical and Applied Genetics, 85: 784-792
Hao Y.F., Liu A.F., Wang Y.H., Feng D.S., Gao J.R., Li X.F., Liu S.B., and
Wang H.G., 2008,
Pm23
: a new allele of
Pm4
located on chromosome
2AL in wheat, Theoretical and Applied Genetics, 117: 1205-1212
Huang X.Q., Hsam S.L.K., and Zeller F.J., 1997, Identification of powdery
mildew resistance genes in common wheat (
Triticum aestivum
L. em
Thell.). IX. Cultivars, land races and breeding lines grown in China,
PlantBreed, 116: 233-238
Li G.P., Chen P.D., Zhang S.Z., Wang X., He Z.H., Zhang Y., Zhao H.,
Huang H.Y., and Zhou X.C., 2007, Effects of the 6VS/6AL
translocation on agronomic traits and dough properties of wheat,
Euphytica, 155: 305- 313
Li H., Chen X., Shi A.N., Kong F.J., Leath S., Murphy J.P., and Jia X., 2005,
Characterization of RAPD markers and RFLP marker linked to
powdery mildew resistant gene derived from different
H.villosa
,
Scientia Agricultura Sinica, 38(3): 439-445
Liu R.H., and Meng J.L., 2003, MapDraw: A microsoft excel Macro for
drawing genetic linkage maps based on given genetic linkage data,
Hereditas, 25(3): 317-321
Liu Z., Sun Q., Ni Z., and Yang T., 1999, Development of SCAR markers
linked to the
Pm21
gene conferring resistance to powdery mildew in
common wheat, Plant Breeding, 118: 215-219
Qi L.L., Cao M.S., Chen P.D., Li W.L., and Liu D.J., 1996, Identification,
mapping and application of polymorphic DNA associated with
resistance gene
Pm21
of wheat, Genome, 39: 191-197
Qi L.L., Chen P.D., Liu D.J., Zhou B., and Zhang S.Z., 1995, The Gene
Pm21
—A new source for resistance to wheat powdery mildew, Acta
Agronomica Sinica, (3): 57-262
Song W., Xie C.J., Du J.K., Xie H., Liu Q., Ni Z.F., Yang T.M., Sun Q.X.,
and Liu Z.Y., 2009, A “one-marker-for-two-genes”approach for
efficient molecular discrimination of
Pm12
and
Pm21
conferring
resistance to powdery mildew in wheat, Mol. Breeding, 23: 357 - 363
Wang C.M., Bie T.D., Chen Q.Z., Cao A.Z., and Chen P.D., 2007,
Development and application of molecular markers specific to
chromosome 6VS of
Haynaldia villosa
,
Acta Agronomica Sinica
,
33(10): 1595-1600
Wang X.Y., Chen P.D., and Zhang S.Z., 2001, Pyramiding and
marker-assisted selection for powdery mildew resistance genes in
common wheat, Acta Genetica Sinica, 28(7): 640-646
Wang Z.Y., Zhao H.M., Hong J.X., Chen L.Y., Zhu J., Li G., Peng Y.K.,
Xie C.J., Liu Z.Y., Sun Q.X., and Yang Z.M., 2007, Identification and
analysis of four novel molecular markers linked to powdery mildew
resistance gene
Pm21
in 6VS chromosome short arm of
Haynaldia
villosa
, Acta Agronomica Sinica, 33(4): 605-611
Yuan Y.Y., Wang Q.Z., Cui F., Zhang J. T., Du B., and Wang H.G., 2010,
Specific loci in genome of wheat milestone parent bima 4 and their
transmission in derivatives, Acta Agronomica Sinica, 36(1): 9-l6
Zong H., Cui F., Bao Y.G., Zhao C.H., Wang Y.H., Du B., Wang Q.Z., and
Wang H.G., 2009, Developing molecular markers for the Rht gene in
dwarfing germplasm line shannong 495, Journal of Triticeae Crops,
29(3): 385-389