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Molecular Plant Breeding 2010, Vol.1 No.3
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Research Article Open Access
Locus R-D1 Conferring Red-Grain-Color in Synthetic Derivative Wheat
Chuanmai 42 Mapped with SSR Markers
Jun Li
1
, Huiting Wei
2
, Xiaorong Hu
1
, Baorong Lu
3
, Wuyun Yang
1
1.Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066
2.Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu, 610066
3.Institute of Biodiversity Science, Fudan University, Shanghai, 200433
Corresponding author email: yangwuyun@yahoo.com.cn;
Authors
Molecular Plant Breeding 2010, Vol.1 No.3 DOI:10.5376/mpb.2010.01.0003
Received: 6 April, 2010
Accepted: 29 June, 2010
Published: 6 September, 2010
This is an Open Access article distributed under the terms of th
which permits unrestricted use, distribution, and reproduction in any
medium, provided the original work is properly cited.
Preferred citation for this article:
Li et al 2010, Locus R-D1 Conferring Red-Grain-Color in Synthetic Derivative Wheat Chuanmai 42 Mapped with SSR Markers, Molecular Plant Breeding
Vol.1 No.3 (DOI:10.5376/mpb.2010.01.0003)
Abstract
Synthetic hexaploid wheat (SHW) (
Triticum durum
×
Aegilops tauschii
) holds a large number of unfavorable traits, of
which, red grain color was important to limit the wheat breeder using SHWs for common wheat improvement. The objectives of this
study were to identify and map the red grain color gene derived from SHW in a Chinese wheat variety Chuanmai 42 by SSR
molecular markers. 1015 individuals in F
2
population derived from the cross of Chuanmai 42 with a red grain color and ChuanW565
(CW565) with white grain color were used for tagging the gene of red grain color. A total of 40 SSR markers on the chromosome 3D
were employed to detect the polymorphism of between the parents, Chuanmai 42 and CW565. 241 recessive individuals in F
2
population derived from the cross (Chuanmai 42×CW565) were employed for allelic test of the red-grain gene by eight polymorphic
markers. The results indicated that the red-grain color gene of Chuanmai 42 derived from the D genome donor of synthetic hexaploid
wheat and controlled by a single dominant gene
R-D1
on the long arm chromosome 3D within an interval of 4.6 flanked by SSR
markers Xgwm3 and Xgwm314, and genetic distance were 2.1 cM, 2.5 cM, respectively. Those two SSR markers can be used in
marker-assisted selection for developing new white grain wheat variety based on the progenies of SHWs or Chuanmai 42 crossed
with white grain color wheat in wheat breeding program.
Keywords
Synthetic hexaploid wheat; Red-grain color;
Aegilops tauschii
; Chuanmai 42; SSR
Background
Grain color is one of important traits affecting flour
yield and quality in wheat. Red grain color of wheat
commonly associated with the development of grain
dormancy and affected brightness of wheat flour due
to contamination of red pigment in milling process
(Flintham, 2000; Warner et al., 2000; Himi et al.,
2002). The most of wheat famers in the world prefer
to grow white grain wheat varieties instead of red one
for the wheat milling quality. Wheat grain color was
controlled by the red seed color genes on the end
region of the long arms of wheat chromosomes 3A,
3B and 3D, respectively (McIntosh et al., 1998). Each
red grain color gene (
R-A1
on 3A,
R-B1
on 3B and
R-D1
on 3D,) was dominant and inherited
monoxenically. The red alleles were assigned as
R-A1b, R-B1b
and
R-D1b
, and the white alleles were
assigned as
R-A1a, R-B1a, and R-D1a
(McIntosh et al.,
1998), a single locus containing the dominant allele
was sufficient to result in red color. The degree of red
color was additive, the intensity of the red color
depended on the number of R alleles, and only those
homozygous recessive at all three genes being white
(
R-A1a, R-B1a
and
R-D1a
).
Synthetic hexaploid wheat (SHW) (
Triticum durum
×
Aegilops tauschii
) was created to explore novel genes
in
T. durum
and
Ae. tauschii
for common wheat
improvement. A plenty of useful traits have been
found in SHW such as disease resistance (Ma et al.,
1995; Kema et al., 1995; Yang et al., 1999), pest
resistance (Eastwood et al., 1991; Thompson et al.,
1999; Hollenhorst and Joppa, 1983;Tyler and Hatchett,
1983), improved zinc efficiency (Cakmak et al., 1999;