Basic HTML Version
Molecular Plant Breeding 2010, Vol.1 No.1
http://mpb.sophiapublisher.com
Page 1 of 10
Research Article Open Access
QTL Analysis of Yield-related Traits using an Advanced Backcross Population
Derived from Common Wild Rice (
Oryza rufipogon
L)
Zhaobin Jing
1,2
, Yanying Qu
3
, Yu Chen
1
, Dajian Pan
1
, Zhilan Fan
1
, Jianyou Chen
1
, Chen Li
1
1.Institute of Rice, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640
2.College of Horticulture, Northwest A &F University and Key Laboratory of Northwest Horticulture Plant Germplasm and Application of Ministry of
Agriculture, Yangling, 712100
3.College of Agriculture, Xinjiang Agricultural University, Urumqi, 830052
Corresponding author email: lic11111@sina.com;
Authors
Molecular Plant Breeding 2010, Vol.1 No.1 DOI:10.5376/mpb.2010.01.0001
Received: 28 July, 2010
Accepted: 6 August, 2010
Published: 6 September, 2010
This is an Open Access article distributed under the terms of th
which permits unrestricted use, distribution, and reproduction in any
medium, provided the original work is properly cited.
Preferred citation for this article:
Jing et al 2010, QTL Analysis of Yield-associated Traits using an Advanced Backcross Population Derived from Common Wild Rice (
Oryza rufipogon
L),
Molecular Plant Breeding Vol.1 No.1 (DOI:10.5376/mpb.2010.01.0001)
Abstract
Wild rice (
Oryza rufipogon
L) is recognized as an important germplasm that has abundant genetic diversity and specific
characters. Many of rice breeders attempt to utilize elite wild rice for rice genetic improvement and breeding program to broaden
genetic background of cultivating rice. Although There are some reports that wild rice germplasms were successfully applied in rice
breeding program, it is definitely depend on what we understand the traits in particular complex traits. In this research the goal was to
identify alleles for yield and yield related components. An advanced backcross BC
3
population was generated by a cross between
recurrent parent named Yuexiangzhan and donor parent named G52
-
9, a wild rice collected in Gaozhou of Guangdong . This
population was evaluated for 10 agronomic traits related to yield and yield related components. Forty three QTLs of ten traits were
detected based on phenotypic data in this study. There are thirty eight QTLs of eight traits detected in continuing two years and
thereinto nine QTLs consistently detected in two continuing years. It is also confirmed that nine QTLs mapped on the same or
adjacent regions conferring the same traits by previous research. Two QTLs,
qGYP
-
2
-
1 and qGYP
-
3
-
1
mapped on chromosome 2
and 3 can increase the yield of Yuexiangzhan by 40.05% and 49.04%, respectively, contributed from wild rice. Furthermore, the
results show that many of detected alleles are likely associated with the trait with wild rice genetic background, which imply that
Gaozhou wild rice should be used as excellent gene donor in rice breeding program.
Keywords
Wild Rice (
Oryza rufipogon
L); Yield-related Trait; Quantitative trait locus (QTL); Advanced backcross population;
AB-QTL analysis
Background
Rice is not only a major food grain in the world but
also is a model plant with a small completely
sequenced whole genome in cereal crops. Modern rice
cultivars have characters of high yielding potentials
and narrow genomic backgrounds due to selection of
traits related to high yield during the past several
decades. Indeed, Enhancing rice yield is still one of
the major goals in rice breeding program so as to meet
the increasing food demands for the growing
populations.
There are abundant genetic diversities formed in the
long-term evolutions from wild rice to cultivating rice.
Wild rice is an important germplasm donor for rice
improvement in rice breeding program. Unfortunately,
Specific and useful genes of the wild rice are only few
available whereas some of characters derived from wild
rice are disappeared or lost in the cultivated rice (Xiao
et al., 1998). The study showed that the number of
alleles in cultivated rice were about 58.2% derived from
of the wild rice among the tested 44 RFLP loci. Further
results demonstrated that the number of allele and
genetic diversity was obviously decreased and reduced.
Some useful alleles even were lost in modem cultivar
of rice due to the breeding pressure of the targeted
selections (Sun et al., 2001).
Most of agronomical traits are quantitative traits
showing normal distributions in phenotype of the