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Legume Genomics and Genetics (online), 2011, Vol. 2, No.3, 14-21
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21
housekeeping gene
NTC
. Drawing amplification curve
and melting curve. Quantitative results were placed on
the coordinate map by taking the average of 3 times
repeats. The relative expression was calculated by
using comparative CT method (
△△
CT) that was, the
relative expression =2
-
△△
CT
=2
-(
△
CT sample -
△
CT control)
=
2
-[(CT sample-CT internal reference) - (CT control -CT internal reference)]
.
Author Contributions
XT, MG, ZY and GP are the persons who designed and
conducted this experiment; LL, LZ and LW finished the data
analysis and paper preparation. LL is the PI of this project
involving in project design, data analysis, and paper
modification. All authors had read and consented the final text.
Acknowledgements
This research is jointly sponsored by the National 863 Project
(No. 2006AA1021F9) and National Special Transgene Projects
(No. 2008ZX08004-002). Authors thank for two anonymous
reviewers with their critical comments. In this paper we
mentioned some chemical and reagent suppliers and
sequencing service providers, that doesn't mean we would like
to recommend or endorse the production of theirs.
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