Page 6 - Legume Genomics and Genetics

Legume Genomics and Genetics (online), 2011, Vol. 2, No.2, 6-13
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Figure 1 Homologous analysis of amino acid sequences among plant ADH family
was measured at 1.12 mg/mL by the BCA method, the
concentration of 6×His-LjADH1 under the optimum
expression conditions.
1.4 Enzymatic activity of fusion LjADH1 protein
We proposed the expressed fusion protein of LjADH
to be a kind of dehydrogenase, therefore we employed
the Vallee and Hoch method to measure enzymatic ac-
tivity(Vallee and Hoch, 1955). The principle of this
method is that NADH and NAD + have their own ma-
ximum absorption peaks at 340 nm and 260 nm, res-
pecttively, so as NAD+ was converted to NADH, the
maximum absorption peaks at 260 nm will be shifted
to 340 nm. The enzymatic activity of LjADH1 was cal-
culated following the formula, U/mg=E340×3.1/(6.2×EW),
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