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Legume Genomics and Genetics (online), 2011, Vol. 2, No.1, 1-5
http://lgg.sophiapublisher.com
1999). Investigation the level of free biotin in various
organs of pea plants found that there is a free biotin in
excess of protein-bound biotin (Hoppe and Zacher,
1985; Somers et al., 1993). In addition, changes in the
amount of streptavdidn binding protein in dry seeds or
in different organs are explained with regard to the
possibility that in plants, as in mammals, biotin plays a
specialized role in cell growth and differentiation
(Hoppe and Zacher, 1985; Somers et al., 1993).
In the present paper streptavidin-alkaline phosphatase
conjugate have been used to detect segregation pattern
and numbers of isotypes of 78 kD streptavidin biotin-
binding proteins (SBP) in the dried pea seeds, ger-
minated seeds, and in different organs including stems,
plumules and roots.
1 Results and discussion
Biosynthesis of biotin has been widely investigated in
bacteria through combined biochemical and genetic
studies (Eisenberg, 1987; Gloeckler et al., 1990). The
purpose of this study was to investigate the changes in
isotype of 78 kD streptavidin binding proteins of
Pisum sativum
L. var Alaska in dormant seeds and
during germination.
Extracted protein from dry pea seeds, embryos of 40
hours or different part of 72 hours embryos including
plumules, stems, and roots were analyzed by 2D-
PAGE, blotted and probed with streptavidin-alkaline
phosphatase conjugate and results shown in (Figure 1,
Figure 2, Figure 3, Figure 4 and Figure 5) respectively.
Figure 1 78 kD (SBP) isotypes in dry pea seeds
Note: The pI values are: 5.66, 5.82, 5.88, 5.91, 5.92, 5.94, 5.96,
5.98, 6.07, 6.50 and 6.55; a, b, c, d, e, f, g, h, k and l refers to
the tenth protruding spots of unseparated isotypes mixture with
pI values 6.07, 6.08, 6.12, 6.14, 6.20, 6.22, 6.25, 6.27, 6.30 and
6.32 respectively
Figure 2 78 kD (SBP) isotypes of embryos of 40 hours ger-
minated pea seeds
Note: The pI values are 5.76, 5.84, 5.89, 5.94, 6.16, 6.30, 6.42,
6.52 and 6.64
Figure 3 78 kD (SBP) isotypes of stems of 72 hours germinated
pea seeds
Note: The pI values are: 5.60, 5.88, 5.97, 6.05, 6.13, 6.18, 6.27,
6.30, 6.39, 6.51 and 6.65
Figure 4 78 kD (SBP) isotypes of plumules of 72 hours germi-
nated pea seeds
Note: The pI values are: 5.78, 5.87, 5.90, 5.98, 6.02, 6.08, 6.30,
6.40, 6.42 and 6.46
Dry pea seeds have a unique isotypes, showing un-
separtated mixture of isotypes with tenth protruding
spots with pI value 6.07, 6.08, 6.12, 6.14, 6.20, 6.22,
6.25, 6.27, 6.30 and 6.32, respectively. The most acidic
and basic isotypes almost separated having a P
I
values
2