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Legume Genomics and Genetics (online), 2010, Vol. 1, No.8, 41-46
http://lgg.sophiapublisher.com
Research Article Open Access
Molecular Mapping and Marker Assisted Selection of Soybean Mosaic Virus Re-
sistance Gene
R
SC
-
12
in Soybean
Ying Ma , Haichao Li , Dagang Wang , Ning Liu , Haijian Zhi
National Key Laboratory for Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement of Nanjing Agricultural University,
Nanjing, 210095
corresponding author email: zhj@njau.edu.cn;
Authors
Legume Genomics and Genetics 2010, Vol.1 No.8 DOI:10.5376/lgg.2010.01.0008
Received: 07 May., 2010
Accepted: 03 Jul., 2010
Published: 20 Nov., 2010
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article as:
Ma et al., 2010, Molecular Mapping and Marker Assisted Selection of Soybean Mosaic Virus Resistance Gene
R
SC-12
in Soybean, Legume Genomics and
Genetics (online), Vol.1 No.8 pp.41-46 (DOI: 10.5376/lgg.2010.01.0008)
Abstract
The P
1
, P
2
, F
1
plants, F
2
population and F
2:3
lines from the cross of Qihuang22×Nannong1138
-
2 were inoculated with
the soybean mosaic virus (SMV) strain SC
-
12 for identification of their resistance in the greenhouse. Qihuang22 and F
1
individuals
were resistant (R), and Nannong1138-2 were susceptible (S). The F
2
population segregated in a ratio of 3 (R):1 (S), and the F
2:3
lines
exhibited a segregation pattern of 1 (R):2 (Segregating):1 (S). These results indicated that a single dominant gene controlled the
resistance to SC
-
12. A F
2
population of Qihuang22×Nannong 1138
-
2 with 219 individuals was constructed for molecular mapping
of resistance gene
R
SC-12
to soybean mosaic virus in soybean. Linkage analysis with bulk segregant analysis (BSA) demonstrated that
the resistance gene
R
SC-12
was located on the linkage group F and linked with seven SSR markers. The order and genetic distance of
markers linked with
R
SC-12
were Sat_297 6.4 cM Sat_234 4.9 cM Sat_154 1.1 cM Satt114 0.7 cM SOYHSP176 1.6 cM Satt334 2.4
cM
R
SC
-
12
6.3cM Sct_033. The marker-assisted selection (MAS) efficiency of SSR markers Satt334 and Sct_033 was evaluated in F
2
,
F
3
and F
4
populations. The results showed that the MAS efficiency of Satt334 and Sct_033 was more than 85%, and that the MAS
efficiency reached as high as 95% when these two markers were co-used. Therefore, the two SSR markers can be used effectively in
selecting for resistance genes
R
SC
-
12
instead of inoculation identification.
Keywords
Soybean; Soybean mosaic virus; Inheritance of resistance; Gene mapping; Marker assisted selection
Background
Soybean mosaic virus (SMV) disease is one of the
most destructive viral diseases in soybean (
Glycine
max
(L.) Merr.) production worldwide, which resulted in
substantial yield losses and seed-quality deterioration.
Planting resistance varieties is the most economical,
effective and environmentally friendly approach for
controlling the disease. However, traditional phenol-
typic selection for varieties resistant to SMV is tim-
econsuming and easily restricted by inoculation and
identification conditions, which might be unavailable
for many breeding agencies. Molecular marker-assisted
selection (MAS) has been proved to be a highly effi-
cient breeding approach to select resistant lines. The
co-dominant simple sequence repeat (SSR) marker, which
has many advantages such as abundant polymorphism,
excellent repeatability, simple and rapid testing and so
on, has been widely used as a tool in MAS breeding
programs.
So far, there were some SSR markers identified for
MAS of soybean cyst nematode (SCN) resistance, such
as Satt309 (Cregan et al., 1999; Wang et al., 2003),
Sat_168 (Cregan et al., 1999), Satt038 (Mudge et al.,
1997; Prabhu et al, 1999), Satt130 (Mudge et al., 1997),
Sat_162 (Meng et al., 2003), Satt610 (Meng et al, 2003)
and so on. However, the researches on soybean resis-
tance to SMV were mainly focused on mapping of the
resistance genes to SMV. Yu et al. (1994), Jeong et al.
(2002) and Hayes et al. (2000) found the molecular
markers closely linked to SMV resistance genes,
Rsv1
,
Rsv3
and
Rsv4
, and located the three resistance genes
on linkage groups F, D1b and B2, respectively. Zhang
et al. (1998, Chinese Science Bulletin, 43(20):
2197-2202) and Wang et al. (2004) mapped the genes
separately controlling resistance to SMV strain Sa,
SC
-
8, SC
-
9, N1, N3. Zheng et al. (2001) mapped the
resistance gene to the SMV3 strain in northeast of
China by using random amplified polymorphic DNA
(RAPD). Researches reported above mostly adopted
molecular markers such as restriction fragment length
polymerphism (RFLP) and RAPD which presented
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