Basic HTML Version
Legume Genomics and Genetics (online), 2010, Vol. 1, No.4, 18-23
http://lgg.sophiapublisher.com
Research Report Open Access
Inheritance Studies of
Pisum sativum
F
1
, F
2
and F
3
Generation Based on Morpholo-
gical Traits and Selection of High Yielding Powdery Mildew Resistant Lines
Nisar M.
1
, Ghafoor A.
2
1. Department of Botany, University of Malakand, NWFP, Pakistan
2.
Institute of Agri-biotechnology and Genetic Resources, National Agricultural Research Centre Islamabad, Pakistan
Co-corresponding author email:
mnshaalpk@yahoo.com
Author
Legume Genomics and Genetics 2010, Vol.1 No.4 DOI:10.5376/lgg.2010.01.0004
Received: 21 Jul., 2010
Accepted: 7 Sep., 2010
Published: 20 Oct., 2010
This article was first published in the Molecular Plant Breeding (Regular Print Version), and here was authorized to redistribute under the terms of the Creative
Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article as:
Nisar M. and Ghafoor A., 2009, Inheritance Studies of
Pisum sativum
F
1
, F
2
and F
3
Generation based Morphological Traits and Selection of High Yielding
Powdery Mildew Resistant Lines, Molecular Plant Breeding, 7(2): 335-340 (DOI: 10.3969/mpb.007.000335)
Abstract
Aim of this study was to determine the inheritance and linkage of six contrasting traits in Pisum sativum F
1
and F
2
generation, and to select high yielding powdery mildew resistant lines in F
3
generation. Two isogenic lines Falloner and 11760-3ER
were selected crossed. All the traits in F
2
segregants, showed 3:1 ratio, which was fit for goodness by χ
2
(p>0.07) method and
indicates monogenic inheritance. Anthocyanin pigmentation linked with flower colour with likely hood ratio 44.31 and with seed
colour at 34.91. While tendril type linked with number of leaflet per leaf at likely hood ratio 33.21. All the three linked qualitative
traits were highly significantly correlated at P<0.00. None of the qualitative trait was linked to powdery mildew disease. In F
3
generation four yields contributing quantitative traits were studied. Among the quantitative traits, total pod weight (49.77%) and
number of seeds/pod (20.01%) showed maximum level of coefficient of variation. In correlation studies, total pod weight pods was
high significantly positively correlated with seed weight, pod width, pod length, number of seed/pod. Similarly, pod length was
highly positively significantly correlated with number of seeds/pod. Based on yield contributing traits and powdery mildew
resistance three lines GN070140-2, GN070143-1 GN070140-0 was selected, which could use as cultivar in future.
Keywords
Peas; Inheritance;
er-1
gene; Analysis; Novel three lines
Background
The genus
Pisum
contains two species,
P. sativum
and
P. fulvum
, both with 2
n
=14 chromosomes. The dried
pea contains 10.9% protein, 1.4% fat, 60.7% carbo-
hydrate, 1.4% crude fiber, and 2.7% ash (Tzitzikas et
al., 2006). Powdery mildew, caused by
Erysiphe pisi
,
is the most wide spread disease of
Pisum sativum
all-
over the world. Powdery mildew causes the crop losses
reaching as high as 25%~86% (Nisar et al., 2006).
Genetic marker provide an attractive alternative to de-
sirable traits selection, making breeding process more
efficient and less resource demanding. Once a genetic
marker that is closely linked to the desirable traits has
been identified, marker assisted (MAS) can be prac-
ticed at early stage of plant development, thus avoi-
ding selection through traits exposure (Rakshit et al.,
2001). MAS can be useful not only for qualitative traits
controlled by a single gene but also for quantitative
traits (Lande and Thompson, 1990). However, the
complex architecture of quantitative traits may limit
the efficiency of MAS for each trait (Ek et al., 2005).
Morphological markers are limited in nature, but their
association with any particular trait is very beneficial
as it neither required sophisticated equipments nor
complicated procedures. Monogenic or oligogenic mor-
phological markers are until recently scientific plant
classification and novel gene identification was based
exclusively on morphological traits (Fondevilla et al.,
2007) some of which may serve as genetic marker
suitable for plant germplasm management (Stanton et
al., 1994).
In Pakistan pea is cultivated under a wide range of agro-
ecological zones. It is cultivated during winter in
plains of Pakistan and during summer in highlands
(Habib and Zamin, 2003). During 2005~2006, the
crop was grown over an area of 90.3 thousands ha
with 52.4 thousand tones production of dry pea, but
the average/hectare yield is very low as compared
with its potential and yield obtained in many other
countries (Anonymou, 2007). Little attention has been
given to varietals improvement of peas and is used as
a marginal crop for cultivation in Pakistan (Bashir and
18