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Table 5 The primer sequence of AFLP
Primer
Sequence
Primer
Sequence
Eco
R
Ⅰ
5
-
CTCGTAGACTGCGTACC
-
3
Mse
Ⅰ
5
-
GACGATGAGTCCTGAG
-
3
3
-
CTGACGCATGGTTAA
-
5
3
-
TACTCAGGACTCAT
-
5
E0
5
-
GACTGCGTACCAATTC
-
3
M0
5
-
GATGAGTCCTGAGTAA
-
3
E1
5
-
GACTGCGTACCAATTCAGG
-
3
M1
5
-
GATGAGTCCTGAGTAACAT
-
3
E2
5
-
GACTGCGTACCAATTCACG
-
3
M2
5
-
GATGAGTCCTGAGTAACTT
-
3
E3
5
-
GACTGCGTACCAATTCAAC
-
3
M3
5
-
GATGAGTCCTGAGTAACAC
-
3
E4
5
-
GACTGCGTACCAATTCACA
-
3
M4
5
-
GATGAGTCCTGAGTAACTA
-
3
E5
5
-
GACTGCGTACCAATTCACC
-
3
M5
5
-
GATGAGTCCTGAGTAACAG
-
3
E6
5
-
GACTGCGTACCAATTCAGC
-
3
M6
5
-
GATGAGTCCTGAGTAACTC
-
3
E7
5
-
GACTGCGTACCAATTCACT
-
3
M7
5
-
GATGAGTCCTGAGTAACAA
-
3
E8
5
-
GACTGCGTACCAATTCAAG
-
3
M8
5
-
GATGAGTCCTGAGTAACTG
-
3
extract genomic DNA from faba bean (Liu et al.,
2007b), and the AFLP Reaction protocols established by
Liu et al. was adopted in this research (Liu et al., 2007a).
3.3 Data Statistics and Analyses
Bands were scored by using 0 or 1, which 1 repre-
sented existing bands and 0 meant no band appeared.
We used mixed model and relational model for occur-
rence frequency of allelic variation to analyze population
genetic structure with the aids of software Structure
2.2. The given parameters of Structure (Burnin, Period
and after Burnin) were fixed 10 000 times, and K
value was set from 1 to 10. Each K value ran 10 times.
The mean values of Var[lnP(D)] corresponded to each
k value and [LnP(D)] were calculated, and then drew
broken line figure to choose the best k value that the
numbers of population genetic structure could be
yielded (Liu et al., 2009). The effective allelic variation,
the polymorphic fragment percentage, the diversity
index of Shannon-weaver and genetic distance were
calculated by Popgen1.32 software; Core collection
was structured by using clustering method (Yang et al.,
2009; Wang et al., 2002).
Authors’ contributions
Yujiao Liu designed and implemented the experiment, and also drafted the
manuscript. Wanwei Hou participated in partial experimental work. Both
authors read and approved the final manuscript.
Acknowledgements
This research was jointly supported by National Key Technology R&D
Program (2007BAD59B05), Special Fund of The Industrial Technology
System Construction of Modem Agriculture and Countermeasures
(nycytx18-G2), and Key Technologies R & D Program of Qinghai Province
(2006-G-126).
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