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Legume Genomics and Genetics (online), 2010, Vol. 1, No.1, 1-6
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genetic diversity existed among populations. The group
four contains 22 populations, of which 14.85% land-
aces, 16.67% foreign varieties and 8.33% released cul-
tivars. The proportion of effective alleles to alleles was
92.50%, and Shannon’s information index was 0.033 2,
The group five contains 17 groups, of which 8.91% land-
races, 16.67% foreign varieties and 16.67% released
cultivars. The proportion of effective alleles to alleles
was 92.50%, and Shannon’s information index was
0.064 5; The group six contains 18 groups, of which
8.91% landraces, 19.44% foreign varieties and 16.67%
released cultivars. The proportion of effective alleles
to alleles was 91.39%, and Shannon’s information index
was 0.077 2; The group seven contains 23 groups, of
which 17.82% landraces and 11.11% foreign varieties.
The proportion of effective alleles to alleles was 92.74%,
and Shannon’s information index was 0.0 598 (Table 3).
1.4 The genetic similarity of tested germplasms of
faba bean
The genetic similarity coefficient of landrance, foreign
varieties and released cultivars were calculated among
149 accessions, which were as 0.948 2 (0.867 1~1.000 0),
0.951 3 (0.884 4~1.000 0), 0.950 3 (0.907 5~0.994 2)
respectively; whereas the genetic distance were as
0.053 5 (0~0.142 7), 0.050 3 (0~0.122 9), and 0.051 1
(0.005 8~0.097 0), respectively (Figure 3). There is
relatively higher genetic distance among landrances,
while lower genetic distance among foreign varieties,
which might be due to size and quantity of population.
Figure 3 The genetic similarity coefficiency and genetic dis-
tance of faba bean germplasm in Qinghai
1.5 Core collection of bean in Qinghai constructed
based on AFLP
The phylogenetic tree of faba bean collection was
established based on the germplasm origins with the
aid of software Popgen V1.32 and MEGA 3.0. The
core collection were assembled based on the phylo-
genetic tree and the targeted germplsams were propor-
tional chosen with closer genetic relationship (Table 4;
Figure 4). Total 40 accessions among 149 accessions
of faba bean were selected as core collection germ-
plasm that accordance rates at least reached 80%
Table 3 The characteristics of genetic information in different groups
Group
Size of Population
Genetic distance
ne/na
Poly-bands
Poly-ratio
Shannon's Information index
Group 1 26
0.013 9
92.96
17
9.83
0.027 8±0.095 7
Group 2 24
0.061 8
89.58
32
18.5
0.065 9±0.160 5
Group 3 20
0.105 8
84.47
47
27.17
0.124 0±0.206 9
Group 4 22
0.025 9
92.50
19
10.98
0.033 2±0.107 7
Group 5 17
0.054 3
89.08
32
18.5
0.064 5±0.151 6
Group 6 18
0.073 9
91.39
31
17.92
0.077 2±0.181 4
Group 7 23
0.055 1
92.74
34
19.65
0.059 8±0.137 4
Table 4 The Comparison between core collection and whole collections
Gene information
Total (149)
Core (40)
Coincidence (%)
T-testing
Number of alleles (na)
1.468 2±0.500 4
1.381 5±0.487 2
94.09
0.087 2
Effective alleles (ne)
1.064 7±0.126 4
1.078 2±0.153 1
98.73
0.025 6
ne/na
72.52
78.05
92.37
/
Poly-bands
81
66
81.48
/
Poly-ratio
46.82
38.15
81.48
/
Coefficiency
0.949 1±0.024 4
0.940 3±0.023 6
99.07
0.040 2
Distance
0.052 6±0.025 9
0.061 9±0.025 2
82.32
0.041 1
Ht
0.030 8±0.012 8
0.058 3±0.010 5
/
0.180 2
Gene diversit
0.050 6±0.087 4
0.103 2±0.164 1
/
0.104 9
Note: t
0.05
=1.96, t
0.01
=2.58
4