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Legume Genomics and Genetics (online), 2010, Vol. 1, No.1, 1-6
http://lgg.sophiapublisher.com
Research Article Open Access
Genetic Diversity of Faba Bean Germplasms in Qinghai and Core Germplasm
Identified based on AFLPAnalysis
Yujiao Liu , Wanwei Hou
Qinghai Academy of Agricultural and Forestry Sciences, Xi’ning, 810016
Corresponding author email: lyujiao2000@yahoo.com.cn;
Author
Legume Genomics and Genetics 2010, Vol.1 No.1 DOI:10.5376/lgg.2010.01.0001
Received: 04 Apr., 2010
Accepted: 06 Aug., 2010
Published: 13 Sep., 2010
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article as:
Yujiao Liu and Wanwei Hou, 2010, Genetic Diversity of Faba Bean Germplasms in Qinghai and Core Germplasm Identified based on AFLP Analysis, Legume
Genomics and Genetics (online), Vol.1 No.1 pp.1-6 (DOI:10.5376/lgg.2010.01.0001)
Abstract
In this study we analyzed the genetic diversities of Qinghai’s faba bean (
Vicia faba
L.) germplasms based on AFLP
techniques. The results showed that the number of effective alleles in landraces, foreign germplasms and released cultivar are 1.065 5±
0.126 3, 1.063 4±0.143 0, and 1.065 9±0.165 1, while the Shannon’s information index are 0.095 4±0.144 9, 0.084 3±0.151 8, 0.074 2±
0.167 9, respectively. The ratio of effective allele of landrace is the lowest (73.73%), while its ploy-ratio is the highest (44.51%).
Whereas the ratio of effective allele of released cultivars is the highest (90.39%), while its ploy-ratio is the lowest (17.92%). The
genetic distance is relatively bigger in the landraces and smaller in the foreign germplasms. Genetic structure analysis revealed that
the 149 faba bean germplasms in Qinghai can be divided into 7 groups. In this study we have selected 40 germplasms as core
germplasm collection which would represent more than 80% genetic information of the tested 149 germplsams based on the genetic
diversity and Nei's gene diversity. It is obvious that the core germplasm collection germplasms might be used as parent materials for
hybrid breeding in faba bean breeding program.
Keywords
Faba bean (
Vicia faba
L.); Faba germplasm; Core germplasm collection; AFLP
Background
AFLP (Amplified Fragment Length Polymorphism) is
a new approach for detecting DNA polymorphism that
was developed by Dutch scientists Zabeau and Vos in
1992. The AFLP procedures are generally as follows:
first the total genomic DNA was digested and then
was amplified by selective PCR. The specific joints
were designed to add on the ends of amplified DNA
fragments. Polymerase chain reaction (PCR) was per-
formed with specific primers that bound the sequences
of the given joints and neighboring restriction enzyme
sites Finally the amplified restriction fragments were
scored on agarose gel electrophoresis (Zhou, 2005).
AFLP is considered as an integration technology whi-
ch possesses both the reliability of RFLP and the con-
venience of RAPD.
1
Qinghai is high altitude plateau province located in
the west of mainland in China, where has a typical
plateau continental climate with obvious vertical dis-
tribution. Faba bean is the traditional crops in Qinghai
which has a long history of cultivation and wider re-
gional distribution. The sowing area of faba bean re-
ached 28 230 hm
2
. reported by the annual statistic
report in Qinghai in 2009. Faba bean is well dis-
tributed at the ranges of the altitude from sea above
1 500 m to 3 000 m. There are abundant faba bean
landrace and germplasms formed due to the domes-
tication and cultivation in the unique ecological en-
vironment. Genetic diversity is recognized as a base
for the germplasm utilization and crop genetical im-
provement. By evaluating the genetic diversity of the
major faba bean germplasms with universal applica-
bility and specificity can predict the variation degree
in the separation progenies derived from pure bree-
ding line. The methods based on the phenotypic and
molecular markers were increasing used for revealing
the diversity of germplasms of beans in the past de-
cade, such as morphological marker used for analy-
zing the genetic diversity of faba germplsms (Wang et
al., 2009; Zhang and Liu, 2007; Liu and Zong, 2008);
AFLP markers were used to study the genetic diver-
sity among 79 of inbred lines of faba bean which ge-
nerated 477 polymorphic fragments (Zeid et al., 2001).
Twenty-eight accessions of faba bean inbred lines
were employed to study the bean size diversity among