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Genomics and Applied Biology, 2011, Vol.2 No.2
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Figure 4 Phylogenetic tree showing evolutionary relationship between cytochrome genes of tomato plants and the others presented in
GenBank
Note: The Neighbor-Joining method (Saitou and Nei 1987) was used to construct the tree; The numbers on the branches represent
bootsrap support for 1,000 replicates; Names refer to the accession number of the nucleotide sequences that encode the
corresponding cytochrome gene
sequence divergence in the two strains.
1.4 The sequence alignment of cytochrome gene in
both resistant and sensitive tomato cultivars
Both amino acids sequences were aligned using
Clustal W program. The results presented in Figure 3
revealed the deletion in two amino acids (Threonine
and Glutamic acid) in cytochrome (FJ712210), which
was isolated from the cultivar TY20. There were also
substitutions in two amino acids in different positions
along the gene.
The first one was in the amino acid position 74, where,
Methionine was substituted with Isoleucine. The
second substitution was in the position 116, where,
Leucine was substituted with Phenylalanine. Our
results agree with what was reported by Gallie et al.
(1998), who observed moderate down regulation of
mRNA with elongation factor 2 (Ef2). Senthil et al
(2005) and Whitham et al (2003) detected significant
induction of mRNA species encoding glutathione-
S-transferase and heat shock protein 90 (HSP90).
Norman and Gray (2001) reported the expression of
the remaining two housekeeping genes, mitochondrion-
encoded cytochrome oxidase subunit I (Cox1) and
cyclophilin (Cph), which remained unaffected during
virus infection.
1.5 Phylogenetic analysis of the ALY-family genes
with
Nicotiana benthamiana
Phylogenetic tree of the DNA and deduced amino
acid sequences of the obtained four ALY-family genes
was constructed with the other ALY genes of the
Nicotiana benthamiana
plants. Data presented in
Figure 5 revealed that genes FJ712208, FJ712213 and
FJ712211 showed similarity with the other
Nicotiana
genes. The identity was 75%, which means that these
genes are completely new. The gene FJ712212 was
considered as an outer group for all the other examined
genes. Canto et al (2006) and Uhrig
et al (2004)
constructed chimeras between two
Arabidopsis
ALY
proteins with different abilities to delocalize P19.
They demonstrated that the C-terminal part of the
ALY RRM domain influences whether the ALY protein