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Genomics and Applied Biology, 2010, Vol.1 No.4
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reference strains and 6 true-negative samples (Table 1).
With regards to the 25 field clinical samples, 19 samples
were detected positive by the multiplex RT-PCR assay, 15
samples by the conventional RT-PCR assay (Table 2).
Table 1 The coincidence of the multiplex RT-PCR and the conventional RT-PCR by detection of the PRRSV reference samples
Multiplex RT-PCR
Conventional RT-PCR
Samples
PN
NN
PN
NN
Sequence analysis
10 reference strains
10
0
10
0
NA
6 true negative samples
0
6
0
6
NA
Coincidence %
100%
100%
100%
100%
/
Note: PN: Positive numbers; NN: Negative numbers; NA: Not analysis
Table 2 The comparison of the multiplex RT-PCR and the conventional RT-PCR by testing 25 clinical samples
Assay result
Origin
M
C
Sequence analysis
Virus isolation
Lung of a dead pig from GSLZ region
+ +
Confirmed
+
Serum of a weaned piglet from GSLZ region
+ +
Confirmed
-
Blood of a dead pig from GSJT region
+ +
Confirmed
-
Lung of an articulo mortis pig from GXYL region
+ +
Confirmed
+
Lung of a dead pig from NXZW region
+ +
NS
+
Heart of a dead pig from NXZW region
+ +
Confirmed
-
Liver of a dead pig from NXZW region
+ -
Confirmed
-
Spleen of a dead pig from NXZW region
- -
Confirmed
-
Kidney of a dead pig from NXZW region
- -
NS
-
Blood of a dead pig from NXZW region
+ -
Confirmed
+
Lung of a weaned piglet from NXPL region
+ +
Confirmed
+
Heart of a dead pig from NXPL region
+ +
Confirmed
-
Liver of a dead pig from NXPL region
- -
NS
-
Spleen of a dead pig from NXPL region
+ -
Confirmed
-
Kidney of a dead pig from NXPL region
- -
NS
-
Blood of an articulo mortis pig from NXPL region
+ -
Confirmed
-
Blood of an articulo mortis pig from NXQTX region
+ +
Confirmed
-
Spleen of a dead pig from NXQTX region
- -
NS
-
Lung of a dead pig from NXQTX region
+ +
Confirmed
+
Lung of a dead pig from NXYCH region
+ +
Confirmed
+
Serum of a dead pig from NXYCH region
+ +
Confirmed
-
Blood of an articulo mortis pig from NXYCH region
+ +
Confirmed
+
Lung of a dead pig from NXHY region
+ +
Confirmed
+
Lung of a dead pig from NXYCH region
+ +
Confirmed
+
Blood of an articulo mortis pig from NXYCH region
- -
NS
-
Note: M: Multiplex RT-PCR; C: Conventional sRT-PCR; * +: Positive;
-
: Negative; “NS”: Not sequenced; “Confirmed” means the sequence
analysis proved; GSLZ is abbreviated from Gansu Lanzhou; GSJT is abbreviated from Gansu Jinta; GXYL is abbreviated from
Gangxi Yulin; NXZW is abbreviated from Ningxia Zhongwei; NXPL is abbreviated from Ningxia Pingluo; NXQTX is abbreviated
from Ningxia Qingtongxia; NXYCH is abbreviated from Ningxia Yingchuan; NXHY is abbreviated from Ningxia Heying