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analysis we included nearly 30 000 gene models
(those that most likely real genes) which were from
the unambiguously mapped sequence scaffolds
along 19 chromosomes of poplar genome (about
86% of the total genome length). Although we
didn’t cover all of the genes and sequences of poplar
genome, the number of genes and genomic
sequences included in this study should be sufficient
to give us a general idea to answer the questions
addressed in this paper.
3 Materials and Methods
The genome sequence and gene information is
obtained
from
poplar
genome
browser
(http://shake.jgi-psf.org/cgi-in/searchGM?db=Poptr1
_1). Gene distribution among chromosome members
was evaluated by the observed number of genes
comparing with their expectations under the Poisson
distribution. The expected gene number
i
in
chromosome
i
would be a sample from a Poisson
distribution,
i
i
i
i
L
mL
/
, where
m
is the
total number of genes detected within chromosome
i
;
i
L
is the length of A,T,C,G reading of
chromosome
i
. The probabilities
)
(
i
i
mp
and
)
(
i
i
mp
were evaluated under the
cumulative Poisson distribution at
=0.05 and
=0.01 significant level. Visual plotting of gene
distribution on each chromosome is drawn by the
Mapchart software (Voorrips, 2002). Chromosome
homology was referred to that revealed by Tuskan et
al. (2006).
P. trichocarpa
EST seuquences were
from Genbank, and EST coverage was summarized
from the gene models predicated in the Vista
browser (http://genome.jgi-psf.org/Poptr1_1/Poptr1_
1.home.html).
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