Basic HTML Version
Bioscience Methods 2012, Vol.3, No.2, 7
-
20
http://bm.sophiapublisher.com
19
at 72
℃
. Further 10 minute final extension was carried
out at 72
℃
. Then gel electrophoresis of PCR product
was done on agarose gel electrophoresis. By using gel
documentation system photograph was taken.
References
Ajit N., 2006, Pulse of Indian Sugar Industry, P: 105, Sugar India Year
Book’-06; Sakal papers Ltd: Kolapur, India, 104-105
Ali A., Siddiqui S.N., and Iqbal F.A.J., 2008, Rapid clonal multiplication
of sugarcane (
Saccharum Officinarum
) through callogenesis and
organogenesis, Pakistan Journal of Botany, 40(1): 123-138
Ali S., Iqbal J., and Khan M.S., 2010, Genotype independent
in vitro
regeneration system in elite varieties of sugarcane, Pakistan Journal of
Botany, 42(6): 3783-3790
Allsopp, P.G., Manners, J.M., Keating B., and Wilson J., 1997, Intensive
sugarcane production: meeting the challenge beyond 2000: proceedings
of the sugar 2000 symposium, Brisbane, Australia, 173-188
Anand, A., 2003, Studies on genetic stability of micropropagated plants
and reintroduction in an endemic and endangered taxon:
Syzgium
travancorium
Gamble, plant biotechnology journal, 5: 201-207
Arencibia A., Prieto D., and Tellez P., 1997, Transgenic sugarcane plants
resistant to stem borer attack, Molecular Breeding, 3(4): 247-255
http://dx.doi.org/10.1023/A:1009616318854
Arvinth, S., Arun S., Selvakesavan R.K., Srikanth N.P., Mukunthan A.,
Kumar A., Premachandran M.N., and Subramonian N., 2010, Genetic
transformation and pyramiding of aprotinin-expressing sugarcane
with cry1Ab for shoot borer (
Chilo infuscatellus
) resistance, Plant Cell
Rep., 29(4): 383-395 http://dx.doi.org/10.1007/s00299-010-0829-5 PMid:
20179936
Ather, A., Khan S., Rehman A., and Nazir M., 2009, Optimization of the
protocols for callus induction, regeneration and acclimatization of
sugarcane cv. Thatta-10, Pakistan Journal of Botany 41: 815-820
Badawy, O.M., Nasir M.I., and Alhendawi R.A., 2008, Response of
sugarcane (Saccharum species hybrid) genotypes to embryogenic callus
induction and in vitro salt stress, Sug. Tech., 10(3): 243-247 http://
dx.doi.org/10.1007/s12355-008-0043-8
Baksha R., Alam R., Karim M.Z., Paul B.S.K., Hossain M.A., Miah M.A.S.,
and Rahman A.B.M.M., 2002, In vitro shoot tip culture of sugarcane
(
Saccharum officinarum
) variety LSD28, Biotechnology, 1(2-4): 67-72
Behera K.K., and Sahoo S., 2009, Rapid in vitro micropropagation of
sugarcane (
Saccharum officinarum
L.) cv-Nayana through callus culture,
Nat. and Sci., 7(4): 7-10
Bennici A., Anzidei M., and Vendramin G.G., 2003, Genetic stability and
uniformity of
Foeniculum vulgate
mill, regenerated plants through
organogenesis and somatic embryogenesis, Plant Science, 166(1):
221-227 http://dx.doi.org/10.1016/j.plantsci.2003.09.010
Bhansali R.R., and Singh K., 1984, Callus and shoot formation from leaf of
sugarcane in tissue culture, Phytomorphol, 167-170
Bower R., and Birch R.G., 1992, Transgenic sugarcane plants via
microprojectile bombardment, Plant J., 2(3): 409-416 http://dx.doi.
org/10.1111/j.1365-313X.1992.00409.x
Burner M.D., 1992, Regeneration and phenotypic variability of plants
cultured
in vitro
from mature sugarcane caryopses, J. Am. Soc. Sug.
Tech., 12: 82-90
Butterfield M.K., 2005, Molecular breeding of sugarcane using DNA
markers and linkage disequilibrium mapping, Proc. Int. Soc. Sugarcane
Technol., 25: 545-548
Chaudhry S.M., and Naseer Z., 2008, Safety of ensiling poultry litter with
sugar cane tops, Pak. J. Agri. Sci., 45(2): 322-366
Cloutier S., and Landry B.S., 1994, Molecular markers applied to plant
tissue culture,
In Vitro
Cell. Develop. Biol., 30(1): 32-39
Comstock J.C., 2000, Smut. In: Rott P., Bailey R.A., Comstock J.C., Croft
B.J., and Salem Saumtally A. (Eds.), A guide to sugarcane diseases,
CIRAD and ISSCT, pp.181-185
Comstock J.C., and Miller J.D., 2004, Yield comparisons: disease-free tissue
culture versus bud-propagated sugarcane plants and healthy versus
yellow leaf infected plants, Journal of the American Society of Sugar
Cane Technologists., 24: 31-40
Dibax R., Alcantara G.B.D., Filho J.C.B., Machado M.P., and Silva
Y.D.O.A.L.L.D., 2011, Plant regeneration of sugarcane cv. RB931003
and RB98710 from somatic embryos and acclimatization, Journal of
Biotechnology and Biodiversity, 5: 32-37
Feuser S., Meier K., Daquinta M., Guerra M.P., and Nodari R.O., 2003,
Genotypic fidelity of micropropagated pineapple (
Ananus comosus
)
plantlets assessed by isozyme and RAPD markers, Plant Cell, Tissue
and Organ Culture, 72: 221-227 http://dx.doi.org/10.1023/A:10223-
21405683
Ford-Lloyd B.V., Sabir A., Newbury H.J., Todd C., and J, 1992,
Determination of genetic stability using isozymes and RFLPs in beet
plants regenerated
in vitro
, Theor. Appl. Genet., 84: 113-117
Gallo-Meagher M., and Irvine J.M., 1996, Herbicide resistant transgenic
sugarcane plants containing the
bar
gene, Crop Sci., 36: 1367-1374
http://dx.doi.org/10.2135/cropsci1996.0011183X003600050047x
Gandonou C., Abrini J., Idaomar M., and Senhaji N.S., 2005, Response of
sugarcane (
Saccharum
sp.) varieties to embryogenic callus induction
and
in vitro
salt stress, Afri J. Biotech. 4: 350-354
Goel Y., Singh V.P., Lal M., and Sharma M.L., 2010,
In vitro
morphogenesis
in leaf sheath explants of sugarcane hybrid var. CoS 99259, Sug. Tech.,
12(2): 172-175 http://dx.doi.org/10.1007/s12355-010-0033-5
Gopitha K., Bhavani L., and Senthilmanickam J., 2010, Effect of the
different auxins and cytokinins in callus induction, shoot, root
regeneration in sugarcane, Int. J. Pharma and Bio. Sci., 1(3): 1-7
Goto S., Thakur R.C., and Ishi K., 1998, Determination of genetic stability
in longterm micropropagated shoots in
Pinus thunbergii
Parl, using
RAPD markers, Plant Cell Rep., 18(3-4): 193-197 http://dx.doi.org/10.
1007/s002990050555
Grivet L., D’Hont A., Roques D., Feldmann P., Lanaud C., and Glaszmann
J.C., 1996, RFLP mapping in cultivated sugarcane (
Saccharum
spp.):
genome organization in a highly polyploid and aneuploid interspecific
hybrid, Genetics, 142(3): 987-1000 PMid:8849904 PMCid:1207035
Gu Y.Q., Wildermuth M.C., and Chakravarthy S., 2002, Tomato transcription
factors pti4, pti5, and pti6 activate defense responses when expressed
in arabidopsis, The Plant Cell, 14(4): 817-831 http://dx.doi.org/10.
1105/tpc.000794 PMCid:150684
Gururaj H., 2001, Sugarcane in agriculture and industry, Prism Books Pvt.
Ltd., India, pp.1472
Heinz D.J., and Mee G.W.P., 1969, Plant differentiation from callus tissue
of
Saccharum
species, Crop Sci., 9: 346 http://dx.doi.org/10.2135/
cropsci1969.0011183X000900030030x
Hidalgo O., Thomma B.J., Carmona E., Borroto C.J., Pujol M., Arencibia A.,
and Lopez J., 2005, Identification of sugarcane genes induced in
disease-resistant somaclones upon inoculation with
Ustilago scitaminea
or
Bipolaris sacchari
, Plant Physiol. Biochem., 43(12): 1115-1121
http://dx.doi.org/10.1016/j.plaphy.2005.07.011 PMid:16386426
Ijaz S., 2012, Engineering sugarcane genome to develop resiatnce against
Colletotrichum falcatum
to control red rot disaese, PhD thesis
University of Agriculture, Faisalabad-Pakistan
Ijaz S., Rana I.A., Khan I.A., and saleem M., 2012, Establishment of an
in
vitro
regeneration system for genetic transformation of selected
sugarcane genotypes, Accepted for publication in Genetics and
Molecular resarch Jouranl
Isabel N., Tremblay L., Michaud M., Tremblay F.M., and Bousquet J., 1993,
RAPDs as an aid to evaluate the genetic integrity of somatic
embryogenesis derived populations of
Picea marina
, Theor. Appl.
Genet., 86(1): 81-87 http://dx.doi.org/10.1007/BF00223811
Jain R., Srivastava S., Singh J., and Gupta P.S., 2005, Assessment of