Molecular Entomology, 2025, Vol.16, No.1, 19-27 http://emtoscipublisher.com/index.php/me 21 Figure 2 Cloth sarong Figure 3 Honey solution and cotton 2.3 Laboratory rearing conditions The environment of the rearing room was kept at a constant temperature, humidity and light cycle. The temperature was set at 26 °C, the relative humidity was 70%~90%, and the light cycle was 10 h of light and 14 h of darkness (10L:14D) per day. The larval culture bottles were placed on the culture racks, arranged in layers, and well ventilated. The feeding and molting of the larvae were checked at a fixed time every day, and the feces were cleaned and fresh corn ears were replaced. The adult cages were placed in the same culture room, and the light was controlled by fluorescent lamps to alternate between light and dark, simulating a circadian rhythm close to nature. 2.4 Experimental methods 2.4.1 Laboratory breeding and observation of corn borer The corn borer larvae collected from the field were placed in multiple culture bottles for feeding, and one ear of fresh corn was placed in each bottle for about 10 larvae to feed. During the feeding process of the larvae, the conditions of molting, replacement of cobs, etc. were observed and recorded regularly until the larvae left the cobs and quietly stopped on the wall of the bottle to pupate after mating. The mature larvae were collected in a clean culture dish covered with filter paper and allowed to pupate in the dish. The collected corn borer pupae were covered with moist filter paper to maintain a certain humidity and placed in an incubator for further cultivation. After the adults emerged, a certain number of male and female adults (about 10 to 15 males and females) were placed in an egg-laying cage together, and tender corn plants were placed in the cage for mating and egg-laying. Check the back of the corn leaves in the cage every day to see if there are egg masses, and record the time and number of egg-laying. Cut the egg masses produced and place them in a small culture dish with moist filter paper to continue hatching the next generation of larvae. The above method was used to establish a continuous breeding population of corn borers in the laboratory, and the survival and development of each generation were recorded.
RkJQdWJsaXNoZXIy MjQ4ODYzNA==