Molecular Entomology 2024, Vol.15, No.2, 52-60 http://emtoscipublisher.com/index.php/me 59 strategies (Tabashnik et al., 2023). Additionally, the development of transgenic sugarcane lines with multiple resistance genes can provide a more durable defense against pests. However, it is essential to address potential non-target effects and ensure that transgenic lines maintain desirable agronomic traits (Srikanth et al., 2011; Wang et al., 2017). Future research should also focus on understanding the ecological impacts of transgenic sugarcane and developing strategies to mitigate any adverse effects on beneficial insects and the environment (Verma et al., 2018; Verma et al., 2022). By adopting a proactive and integrated approach, the long-term sustainability of genetically engineered sugarcane for pest resistance can be achieved. Acknowledgments I sincerely appreciates the valuable opinions and suggestions provided by the three anonymous reviewers, whose meticulous review greatly helped me improve the quality of this manuscript. Conflict of Interest Disclosure The author affirms that this research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. References Anderson J., Ellsworth P., Faria J., Head G., Owen M., Pilcher C., Shelton A., and Meissle M., 2019, Genetically engineered crops: importance of diversified integrated pest management for agricultural sustainability, Frontiers in Bioengineering and Biotechnology, 7: 24. https://doi.org/10.3389/fbioe.2019.00024 Arruda P., 2012, Genetically modified sugarcane for bioenergy generation, Current Opinion in Biotechnology, 23(3): 315-322. https://doi.org/10.1016/j.copbio.2011.10.012 Birch R., 1996, New gene technologies and their potential value for sugarcane, Outlook on Agriculture, 25: 219-226. https://doi.org/10.1177/003072709602500403 Boulter D., 1989, Genetic engineering of plants for insect resistance, Outlook on Agriculture, 18: 2-6. https://doi.org/10.1177/003072708901800101 Budeguer F., Enrique R., Perera M., Racedo J., Castagnaro A., Noguera A., and Welin B., 2021, Genetic transformation of sugarcane, current status and future prospects, Frontiers in Plant Science, 12: 768609. https://doi.org/10.3389/fpls.2021.768609 Chung S., Feng H., and Jander G., 2021, Engineering pest tolerance through plant-mediated RNA interference, Current Opinion in Plant Biology, 60: 102029. https://doi.org/10.1016/j.pbi.2021.102029 Dessoky E., Ismail R., Elarabi N., Abdelhadi A., and Abdallah N., 2020, Improvement of sugarcane for borer resistance using Agrobacterium mediated transformation of cry1Ac gene, GM Crops and Food, 12: 47-56. https://doi.org/10.1080/21645698.2020.1809318 Eakteiman G., Moses-Koch R., Moshitzky P., Mestre-Rincon N., Vassão D., Luck K., Sertchook R., Malka O., and Morin S., 2018, Targeting detoxification genes by phloem-mediated RNAi: A new approach for controlling phloem-feeding insect pests, Insect Biochemistry and Molecular Biology, 100: 10-21. https://doi.org/10.1016/j.ibmb.2018.05.008 Fabrick J., LeRoy D., Mathew L., Wu Y., Unnithan G., Yelich A., Carrière Y., Li X., and Tabashnik B., 2021, CRISPR-mediated mutations in the ABC transporter gene ABCA2 confer pink bollworm resistance to Bt toxin Cry2Ab, Scientific Reports, 11(1): 10377. https://doi.org/10.1038/s41598-021-89771-7 Guo Z., Sun D., Kang S., Zhou J., Gong L., Qin J., Guo L., Zhu L., Bai Y., Luo L., and Zhang Y., 2019, CRISPR/Cas9-mediated knockout of both the PxABCC2 and PxABCC3 genes confers high-level resistance to Bacillus thuringiensis Cry1Ac toxin in the diamondback moth, Plutella xylostella (L.), Insect Biochemistry and Molecular Biology, 107: 31-38. https://doi.org/10.1016/j.ibmb.2019.01.009 Halder K., Chaudhuri A., Abdin M., Majee M., and Datta A., 2022, RNA interference for improving disease resistance in plants and its relevance in this clustered regularly interspaced short palindromic repeats-dominated era in terms of dsRNA-based biopesticides, Frontiers in Plant Science, 13: 885128. https://doi.org/10.3389/fpls.2022.885128 Hilder V., Gatehouse A., Sheerman S., Barker R., and Boulter D., 1987, A novel mechanism of insect resistance engineered into tobacco, Nature, 330: 160-163. https://doi.org/10.1038/330160A0 Iqbal A., Khan R., Khan M., Gul K., Jalil F., Shah D., Rahman H., and Ahmed T., 2021, Genetic engineering approaches for enhanced insect pest resistance in sugarcane, Molecular Biotechnology, 63: 557-568. https://doi.org/10.1007/s12033-021-00328-5 Krishna S., Chandar S., Ravi M., Valarmathi R., Lakshmi K., Prathima P., Manimekalai R., Viswanathan R., Hemaprabha G., and Appunu C., 2023, Transgene-free genome editing for biotic and abiotic stress resistance in sugarcane: prospects and challenges, Agronomy, 13(4): 1000. https://doi.org/10.3390/agronomy13041000
RkJQdWJsaXNoZXIy MjQ4ODYzNA==