Journal of Mosquito Research 2024, Vol.14, No.1, 34-48 http://emtoscipublisher.com/index.php/jmr 42 communities in the adult stage. Fungal treatment (red column) showed significantly lower levels than non-fungal treatment (blue column), indicating that fungal treatment has a significant inhibitory effect on the microbial community in the adult stage. The SCML count of the non-fungal treatment group was significantly higher than that of the fungal treatment group, and the difference was significant. Figure 3 Rarefaction analysis for each sample (Wang et al., 2011) Note: OTUs at 5% distance for each site was used to calculate rarefaction curves; Sugar: mosquitoes were fed with sugar meal; PBM: post blood meal The number of microbial communities in the larval stage is significantly higher than that in the adult stage, indicating that the number of microbial communities will significantly decrease with changes in insect development stage. Fungal treatment significantly inhibited the number of microbial communities during the adult stage, while the effect was relatively small during the larval stage. This may be because fungi have a stronger pathogenic or inhibitory effect on the microbial community during the adult stage. An asterisk (*) is marked in the figure to indicate statistical significance: there is a significant difference in SCML counts between the larval and adult stages (indicated by the horizontal line above the asterisk). There is a significant difference between fungal and non-fungal treatments during the adult stage. This bar chart indicates significant differences in the number of microbial communities at different developmental stages and under different treatments. The number of microbial communities in the larval stage is much higher than that in the adult stage, while fungal treatment has a significant inhibitory effect on the microbial communities in the adult stage. This difference may be related to changes in insect physiological conditions and microbial colonization environment, indicating that the adaptability and resistance of microbial communities to insects are of great significance at different developmental stages and treatment conditions. In summary, the impact of gut microbiota on mosquito host seeking behavior provides a new perspective and method for controlling mosquito populations and preventing mosquito borne diseases. Future research needs to further explore the specific mechanisms by which different microorganisms affect mosquito behavior, as well as how to effectively apply this strategy in practical environments. 6.2 Case two: microbial regulation of mosquito reproductive behavior The reproductive behavior of mosquitoes is a crucial aspect of their life cycle, directly related to the maintenance of the population and the ability to spread diseases. In the natural environment, the reproductive behavior of
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