Journal of Mosquito Research 2024, Vol.14, No.1, 34-48 http://emtoscipublisher.com/index.php/jmr 39 approach. By selectively introducing or increasing certain microbial species, regulation of mosquito populations can be achieved, reducing their numbers and slowing down the rate of disease transmission. Entomopathogenic microorganisms, such as Bacillus thuringiensis and Bacillus sphaericus, are microorganisms that are widely used in eco-friendly mosquito control. The insecticidal crystal proteins produced by these microorganisms are highly selective for mosquito larvae and have a low impact on other non-target organisms, which is in line with the concept of eco-friendly control. Biocides often contain components of microbial origin, such as mosquito-specific insect biocides. These microbial sources can be applied to mosquito breeding sites by injection or spraying to provide control. Compared with chemical pesticides, these biopesticides have less environmental impact and do not produce excessive negative effects on non-target organisms. 4.3 Application of genetic modification and mosquito tolerance Through genetic modification technology, scientists can introduce microbial resistance genes that are harmful to mosquitoes into their genomes (Demirak et al., 2022). For example, introducing genes that are resistant to pathogens that transmit diseases in mosquitoes into mosquitoes gives them a higher level of resistance when infected with the pathogens, thus slowing down the spread of the diseases. Genetic modification also allows for the regulation of interactions between mosquitoes and microbes. Scientists can adjust gene expression in the mosquito's immune system to make it more likely to establish symbiotic relationships with certain microbes, or to increase the mosquito's tolerance of certain beneficial microbes. This helps maintain a state of equilibrium in the ecosystem. When conducting research on genetically engineered mosquitoes, scientists must fully consider their potential impact on the ecosystem. Microorganisms, as an important part of the mosquito ecosystem, and their interactions with genetically engineered mosquitoes need to be studied in depth to ensure that the release of genetically engineered mosquitoes has no unforeseen negative impacts on the ecological balance and microbial communities. When conducting research on genetically engineered mosquitoes, scientists must fully consider their potential impact on the ecosystem. Microorganisms, as an important part of the mosquito ecosystem, and their interactions with genetically engineered mosquitoes need to be studied in depth to ensure that the release of genetically engineered mosquitoes has no unforeseen negative impacts on the ecological balance and microbial communities. The application of microorganisms in mosquito control exhibits a multilayered character, including both applications in eco-friendly mosquito control methods and potential applications in the study of genetically engineered mosquitoes. By utilizing microorganisms for mosquito ecological management, precise control of mosquito populations can be achieved, slowing down the rate of disease transmission. Meanwhile, genetic engineering technology provides new means for mosquito control, and the potential application of microorganisms in this field can help realize more precise and environmentally friendly mosquito control strategies. Future research should continue to dig deeper into the mechanism of microorganisms in mosquito control and actively promote the application of microorganisms in mosquito control in practice. 5 Research Methods and Techniques 5.1 Microbial identification techniques Microorganisms play a key role in mosquito behavioral studies, and advanced microbial identification techniques need to be applied in order to gain insights into the effects of microorganisms on mosquito behavior (Chen et al., 2022). Some commonly used microbial identification techniques will be described below to provide a viable approach in mosquito behavioral studies.
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