Journal of Mosquito Research 2024, Vol.14, No.1, 18-25 http://emtoscipublisher.com/index.php/jmr 21 Table 2 summarizes the results following virus delivery to squirrel monkeys via cartons of 15 mosquitoes, including viremia, virus transmission to mosquitoes, and neutralizing antibody response. The table records individual and control group data for Dengue virus (DENV) and Zika virus (ZIKV), along with corresponding experimental data. Results indicate that squirrel monkeys treated with ZIKV exhibited higher peak viremia and mosquito infection rates. For instance, squirrel monkeys treated with ZIKV showed a viremia duration of 3 to 5 days and higher mosquito transmission rates, reflecting ZIKV's higher transmission potential. Moreover, PRNT80 results suggest that the presence of the virus in the host does not always lead to effective mosquito infection, reflecting the complex relationship between viral exposure in the host and immune protection. Table 2 Virus delivered to squirrel monkeys via cartons of 15 mosquitoes and subsequent viremia, transmission to mosquitoes, and neutralizing antibody response Figure 3 illustrates the replication and transmission of wild-type Dengue virus serotype 2 (DENV-2) in ancestral hosts (rhesus monkeys) and new hosts (squirrel monkeys). In both of these primate species, DENV-2 replication levels were low, and transmission to Aedes albopictus mosquitoes occurred infrequently. When viremia was not detected in rhesus and squirrel monkeys, their data points for mosquito infection were placed below the limit of detection (LOD) at an arbitrary position for ease of observation. Viremia was monitored by direct titration of sera and also by titration after one passage of the serum; transmission monitoring involved allowing 10 to 15 uninfected mosquitoes to feed on each monkey. Typically, the number of mosquitoes that fed was less than the total number of mosquitoes prepared.
RkJQdWJsaXNoZXIy MjQ4ODY0NQ==