International Journal of Molecular Evolution and Biodiversity, 2025, Vol.15, No.2, 99-110 http://ecoevopublisher.com/index.php/ijmeb 105 complex variable relationships. Shi et al. (2024b) successfully established an association model between volatile oil content and key phenotypic traits through PCA analysis, and screened out excellent populations with excellent medicinal value and ecological adaptability. Geographic ecotype research reveals the interactive effect of environment and genotype. Linderae populations in specific areas of Qingchuan County not only show high volatile oil content, but also maintain rich genetic diversity (Gu et al., 2010; Peng et al., 2020). This excellent characteristic formed by the synergistic effect of "genotype × environment" can be transformed into industrial advantages through targeted breeding, providing an important material basis for the efficient utilization of Linderae resources. 5.3 Screening of germplasm with superior adaptability In the screening of environmentally adaptable resources, the focus is on the comprehensive evaluation of major stress resistance traits such as drought resistance, cold resistance and salt tolerance. The research strategy usually combines gene expression profile analysis with physiological response experiments to systematically reveal the potential of germplasm to adapt to adversity from multiple dimensions. The results showed that some genes closely related to osmotic regulation and key regulatory factors in the ABA signaling pathway showed a significant high expression trend in high-adaptability materials. The existence of such expression patterns gives it an important reference value as a molecular marker for resistance screening (Xiong et al., 2020). Lindera aggregata populations show significant differences in environmental adaptability along the altitude gradient: high-altitude populations have outstanding cold tolerance, while low-altitude populations show stronger drought resistance. This ecotype differentiation is the result of long-term natural selection and provides valuable genetic resources for targeted breeding. By accurately matching germplasm characteristics with the environmental conditions of the cultivation site, the success rate and stability of artificial cultivation of Lindera aggregata can be significantly improved (Guo et al., 2020). 6 Development and Utilization of Lindera aggregata Germplasm Resources in Qingchuan County 6.1 Breeding and promotion of superior germplasm The development of Lindera aggregata germplasm resources in Qingchuan requires the establishment of an efficient modern breeding technology platform. The application of biotechnologies such as tissue culture and asexual reproduction has broken through the limitations of traditional breeding methods (Bh et al., 2017; Salgotra and Chauhan, 2023). These innovative technologies can not only maintain the genetic stability of excellent traits, but also greatly improve the reproduction efficiency, providing a reliable guarantee for the large-scale promotion of high-quality germplasm (Shi et al., 2024b). Industrial promotion should focus on selecting high-quality germplasm with high medicinal value and wide adaptability. Establishing a germplasm certification system and a regional brand strategy is an effective way to enhance market competitiveness, which can not only ensure the quality of medicinal materials, but also achieve sustainable utilization of resources (Salleh, 2020). At present, classic prescriptions with Qingchuan Linderae as the main drug, such as "Suquan Pills" for the treatment of nocturia and "Zhengqi Tianxiang Powder" for regulating gynecological diseases, are still widely used in clinical practice (Table 1) (Huang et al., 2023), demonstrating its important medicinal value. 6.2 Conservation measures for germplasm resources Building a complete germplasm preservation system is the fundamental guarantee for maintaining the genetic diversity of Linderae obesa. Modern germplasm resource banks and gene banks have stored rich genetic materials for future breeding and scientific research by cryopreserving seeds, in vitro tissues and DNA samples (Rao and Hodgkin, 2004; Govindaraj et al., 2015; Carvalho et al., 2019). With the help of molecular marker technologies such as SSR and SNP, researchers can accurately classify the stored resources to ensure the complete preservation of genetic diversity (Guan et al., 2020).
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